PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

Mohammad M Al-Sabi; Peter Deplazes; Pia Webster; Jakob Willesen; R.K. Davidson; Christian Moliin Outzen Kapel

doi:10.1007/s00436-010-1847-5

PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

Mohammad M Al-Sabi, Peter Deplazes, Pia Webster, Jakob Willesen, R.K. Davidson, Christian Moliin Outzen Kapel

51 Citations (Scopus)

Abstract

The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

Original language	English
Journal	Parasitology Research
Volume	107
Issue number	1
Pages (from-to)	135-140
Number of pages	6
ISSN	0932-0113
DOIs	https://doi.org/10.1007/s00436-010-1847-5
Publication status	Published - Jun 2010

Access to Document

10.1007/s00436-010-1847-5

Cite this

@article{453c39ec91404a348da575f621df4282,

title = "PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes",

abstract = "The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.",

author = "Al-Sabi, {Mohammad M} and Peter Deplazes and Pia Webster and Jakob Willesen and R.K. Davidson and Kapel, {Christian Moliin Outzen}",

year = "2010",

month = jun,

doi = "10.1007/s00436-010-1847-5",

language = "English",

volume = "107",

pages = "135--140",

journal = "Parasitology Research",

issn = "0932-0113",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

AU - Al-Sabi, Mohammad M

AU - Deplazes, Peter

AU - Webster, Pia

AU - Willesen, Jakob

AU - Davidson, R.K.

AU - Kapel, Christian Moliin Outzen

PY - 2010/6

Y1 - 2010/6

N2 - The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

AB - The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

U2 - 10.1007/s00436-010-1847-5

DO - 10.1007/s00436-010-1847-5

M3 - Journal article

C2 - 20372926

SN - 0932-0113

VL - 107

SP - 135

EP - 140

JO - Parasitology Research

JF - Parasitology Research

IS - 1

ER -

PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

Abstract

Access to Document

Fingerprint

Cite this