PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

Mohammad M Al-Sabi; Peter Deplazes; Pia Webster; Jakob Willesen; R.K. Davidson; Christian Moliin Outzen Kapel

doi:10.1007/s00436-010-1847-5

PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

Mohammad M Al-Sabi, Peter Deplazes, Pia Webster, Jakob Willesen, R.K. Davidson, Christian Moliin Outzen Kapel

51 Citationer (Scopus)

Abstract

The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

Originalsprog	Engelsk
Tidsskrift	Parasitology Research
Vol/bind	107
Udgave nummer	1
Sider (fra-til)	135-140
Antal sider	6
ISSN	0932-0113
DOI	https://doi.org/10.1007/s00436-010-1847-5
Status	Udgivet - jun. 2010

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10.1007/s00436-010-1847-5

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AU - Al-Sabi, Mohammad M

AU - Deplazes, Peter

AU - Webster, Pia

AU - Willesen, Jakob

AU - Davidson, R.K.

AU - Kapel, Christian Moliin Outzen

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AB - The cardiovascular nematode Angiostrongylus vasorum is spreading in the fox and dog populations of northern Europe. A. vasorum can result in severe clinical manifestations in dogs; therefore, specific diagnosis is crucial for assessing its prevalence. In the present study, faecal samples from foxes and domestic dogs were tested by a new polymerase chain reaction (PCR) targeting the second internal transcribed region of the ribosomal DNA (ITS2) of A. vasorum. Initial isolation of faecal larvae by sieving facilitated the processing of larger sample volumes and allowed for the recovery of dead larvae from frozen samples. The sieve-PCR method enabled the identification of a single larva per 2 g of faecal sample and did not amplify DNA of a range of canine helminths, thus presenting a non-invasive tool for wildlife surveillance and for confirmative diagnosis in dogs.

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DO - 10.1007/s00436-010-1847-5

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PCR detection of Angiostrongylus vasorum in faecal samples of dogs and foxes

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