TY - JOUR
T1 - Near-infrared spectroscopy using a spercontinuum laser
T2 - application to long wavelength transmission spectra of barley endosperm and oil
AU - Ringsted, Tine
AU - Dupont, Sune Hoveroust
AU - Ramsay, Jacob Søndergaard
AU - Jespersen, Birthe P Møller
AU - Sørensen, Klavs Martin
AU - Keiding, Søren Rud
AU - Engelsen, Søren Balling
PY - 2016/7/1
Y1 - 2016/7/1
N2 - The supercontinuum laser is a new type of light source, which combines the collimation and intensity of a laser with the broad spectral region of a lamp. Using such a source therefore makes it possible to focus the light onto small sample areas without losing intensity and thus facilitate either rapid or high-intensity measurements. Single seed transmission analysis in the long wavelength (LW) near-infrared (NIR) region is one area that might benefit from a brighter light source such as the supercontinuum laser. This study is aimed at building an experimental spectrometer consisting of a supercontinuum laser source and a dispersive monochromator in order to investigate its capability to measure the barley endosperm using transmission experiments in the LW NIR region. So far, barley and wheat seeds have only been studied using NIR transmission in the short wavelength region up to 1100 nm. However, the region in the range of 2260-2380 nm has previously shown to be particularly useful in differentiating barley phenotypes using NIR spectroscopy in reflectance mode. In the present study, 350 seeds (consisting of 70 seeds from each of five barley genotypes) in 1 mm slices were measured by NIR transmission in the range of 2235-2381 nm and oils from the same five barley genotypes were measured in a cuvette with a 1 mm path length in the range of 2003-2497 nm. The spectra of the barley seeds could be classified according to genotypes by principal component analysis; and spectral covariances with reference analysis of moisture, β-glucan, starch, protein and lipid were established. The spectral variations of the barley oils were compared to the fatty acid compositions as measured using gas chromotography-mass spectrometry (GC-MS).
AB - The supercontinuum laser is a new type of light source, which combines the collimation and intensity of a laser with the broad spectral region of a lamp. Using such a source therefore makes it possible to focus the light onto small sample areas without losing intensity and thus facilitate either rapid or high-intensity measurements. Single seed transmission analysis in the long wavelength (LW) near-infrared (NIR) region is one area that might benefit from a brighter light source such as the supercontinuum laser. This study is aimed at building an experimental spectrometer consisting of a supercontinuum laser source and a dispersive monochromator in order to investigate its capability to measure the barley endosperm using transmission experiments in the LW NIR region. So far, barley and wheat seeds have only been studied using NIR transmission in the short wavelength region up to 1100 nm. However, the region in the range of 2260-2380 nm has previously shown to be particularly useful in differentiating barley phenotypes using NIR spectroscopy in reflectance mode. In the present study, 350 seeds (consisting of 70 seeds from each of five barley genotypes) in 1 mm slices were measured by NIR transmission in the range of 2235-2381 nm and oils from the same five barley genotypes were measured in a cuvette with a 1 mm path length in the range of 2003-2497 nm. The spectra of the barley seeds could be classified according to genotypes by principal component analysis; and spectral covariances with reference analysis of moisture, β-glucan, starch, protein and lipid were established. The spectral variations of the barley oils were compared to the fatty acid compositions as measured using gas chromotography-mass spectrometry (GC-MS).
KW - Faculty of Science
KW - Barley seed
KW - Beta-glucan
KW - Food
KW - near-infrared spectroscopy
KW - NIR
KW - near-infrared transmission spectroscopy
KW - NIT
KW - Single seed
KW - supercontinuum laser
KW - Barley seed
KW - beta-glucan
KW - food
KW - near-infrared spectroscopy
KW - NIR
KW - near-infrared transmission spectroscopy
KW - NIT
KW - single seed
KW - supercontinuum laser
U2 - 10.1177/0003702816652361
DO - 10.1177/0003702816652361
M3 - Journal article
C2 - 27340221
SN - 0003-7028
VL - 70
SP - 1176
EP - 1185
JO - Applied Spectroscopy
JF - Applied Spectroscopy
IS - 7
ER -