Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.

Annette Iversen; Giuseppe Cazzamali; Michael Williamson; Frank Hauser; Cornelis J P Grimmelikhuijzen

doi:10.1016/S0006-291X(02)02709-2

Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.

Annette Iversen, Giuseppe Cazzamali, Michael Williamson, Frank Hauser, Cornelis J P Grimmelikhuijzen

Cell and Neurobiology

88 Citations (Scopus)

Abstract

The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.

Original language	English
Journal	Biochemical and Biophysical Research Communications
Volume	299
Issue number	4
Pages (from-to)	628-33
Number of pages	5
ISSN	0006-291X
DOIs	https://doi.org/10.1016/S0006-291X(02)02709-2
Publication status	Published - 2002

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@article{00de7c20ec2811dcbee902004c4f4f50,

title = "Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.",

abstract = "The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.",

author = "Annette Iversen and Giuseppe Cazzamali and Michael Williamson and Frank Hauser and Grimmelikhuijzen, {Cornelis J P}",

note = "Keywords: Amino Acid Sequence; Animals; Anopheles; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Neuropeptides; Open Reading Frames; Receptors, Cell Surface; Sequence Alignment",

year = "2002",

doi = "10.1016/S0006-291X(02)02709-2",

language = "English",

volume = "299",

pages = "628--33",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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T1 - Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.

AU - Iversen, Annette

AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Hauser, Frank

AU - Grimmelikhuijzen, Cornelis J P

N1 - Keywords: Amino Acid Sequence; Animals; Anopheles; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Neuropeptides; Open Reading Frames; Receptors, Cell Surface; Sequence Alignment

PY - 2002

Y1 - 2002

N2 - The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.

AB - The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.

U2 - 10.1016/S0006-291X(02)02709-2

DO - 10.1016/S0006-291X(02)02709-2

M3 - Journal article

C2 - 12459185

SN - 0006-291X

VL - 299

SP - 628

EP - 633

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.

Abstract

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