TY - JOUR
T1 - Molecular cloning and functional expression of a Drosophila receptor for the neuropeptides capa-1 and -2.
AU - Iversen, Annette
AU - Cazzamali, Giuseppe
AU - Williamson, Michael
AU - Hauser, Frank
AU - Grimmelikhuijzen, Cornelis J P
N1 - Keywords: Amino Acid Sequence; Animals; Anopheles; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; Drosophila Proteins; Drosophila melanogaster; Molecular Sequence Data; Neuropeptides; Open Reading Frames; Receptors, Cell Surface; Sequence Alignment
PY - 2002
Y1 - 2002
N2 - The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.
AB - The Drosophila Genome Project website contains an annotated gene (CG14575) for a G protein-coupled receptor. We cloned this receptor and found that the cloned cDNA did not correspond to the annotated gene; it partly contained different exons and additional exons located at the 5(')-end of the annotated gene. We expressed the coding part of the cloned cDNA in Chinese hamster ovary cells and found that the receptor was activated by two neuropeptides, capa-1 and -2, encoded by the Drosophila capability gene. Database searches led to the identification of a similar receptor in the genome from the malaria mosquito Anopheles gambiae (58% amino acid residue identities; 76% conserved residues; and 5 introns at identical positions within the two insect genes). Because capa-1 and -2 and related insect neuropeptides stimulate fluid secretion in insect Malpighian (renal) tubules, the identification of this first insect capa receptor will advance our knowledge on insect renal function.
U2 - 10.1016/S0006-291X(02)02709-2
DO - 10.1016/S0006-291X(02)02709-2
M3 - Journal article
C2 - 12459185
SN - 0006-291X
VL - 299
SP - 628
EP - 633
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -
