Localization of checkpoint and repair proteins in eukaryotes

TY - JOUR

T1 - Localization of checkpoint and repair proteins in eukaryotes

AU - Lisby, Michael

AU - Rothstein, Rodney

N1 - Keywords: Double-strand break; Replication; DNA damage; Checkpoint; Foci; Homologous recombination; Non-homologous end-joining; Repair center

PY - 2005

Y1 - 2005

N2 - In eukaryotes, the cellular response to DNA damage depends on the type of DNA structure being recognized by the checkpoint and repair machinery. DNA ends and single-stranded DNA are hallmarks of double-strand breaks and replication stress. These two structures are recognized by distinct sets of proteins, which are reorganized into a focal assembly at the lesion. Moreover, the composition of these foci is coordinated with cell cycle progression, reflecting the favoring of end-joining in the G1 phase and homologous recombination in S and G2. The assembly of proteins at sites of DNA damage is largely controlled by a network of protein-protein interactions, with the Mre11 complex initiating assembly at DNA ends and replication protein A directing recruitment to single-stranded DNA. This review summarizes current knowledge on the cellular organization of DSB repair and checkpoint proteins focusing on budding yeast and mammalian cells.

AB - In eukaryotes, the cellular response to DNA damage depends on the type of DNA structure being recognized by the checkpoint and repair machinery. DNA ends and single-stranded DNA are hallmarks of double-strand breaks and replication stress. These two structures are recognized by distinct sets of proteins, which are reorganized into a focal assembly at the lesion. Moreover, the composition of these foci is coordinated with cell cycle progression, reflecting the favoring of end-joining in the G1 phase and homologous recombination in S and G2. The assembly of proteins at sites of DNA damage is largely controlled by a network of protein-protein interactions, with the Mre11 complex initiating assembly at DNA ends and replication protein A directing recruitment to single-stranded DNA. This review summarizes current knowledge on the cellular organization of DSB repair and checkpoint proteins focusing on budding yeast and mammalian cells.

U2 - 10.1016/j.biochi.2004.10.023

DO - 10.1016/j.biochi.2004.10.023

M3 - Review

C2 - 15989975

SN - 0300-9084

VL - 87

SP - 579

EP - 589

JO - Biochimie

JF - Biochimie

IS - 7

ER -