Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Louise Kruse Johansen; Tine Moesgaard Iburg; Ole Lerberg Nielsen; Páll S. Leifsson; Kirstin Dahl-Pedersen; Janne Koch; Dorte Frees; Bent Aalbæk; Peter M. H. Heegaard; Henrik Michael Elvang Jensen

doi:10.1016/j.prostaglandins.2012.01.002

Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Louise Kruse Johansen, Tine Moesgaard Iburg, Ole Lerberg Nielsen, Páll S. Leifsson, Kirstin Dahl-Pedersen, Janne Koch, Dorte Frees, Bent Aalbæk, Peter M. H. Heegaard, Henrik Michael Elvang Jensen

6 Citations (Scopus)

Abstract

It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6 h, 12 h, 24 h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.

Original language	English
Journal	Prostaglandins & Other Lipid Mediators
Volume	97
Issue number	3-4
Pages (from-to)	103-108
Number of pages	6
ISSN	1098-8823
DOIs	https://doi.org/10.1016/j.prostaglandins.2012.01.002
Publication status	Published - Mar 2012

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Johansen, L. K., Iburg, T. M., Nielsen, O. L., Leifsson, P. S., Dahl-Pedersen, K., Koch, J., Frees, D., Aalbæk, B., Heegaard, P. M. H., & Jensen, H. M. E. (2012). Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis. Prostaglandins & Other Lipid Mediators, 97(3-4), 103-108. https://doi.org/10.1016/j.prostaglandins.2012.01.002

@article{5412f1258f144d8da2f558913aadfe1a,

title = "Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis",

abstract = "It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6 h, 12 h, 24 h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.",

author = "Johansen, {Louise Kruse} and Iburg, {Tine Moesgaard} and Nielsen, {Ole Lerberg} and Leifsson, {P{\'a}ll S.} and Kirstin Dahl-Pedersen and Janne Koch and Dorte Frees and Bent Aalb{\ae}k and Heegaard, {Peter M. H.} and Jensen, {Henrik Michael Elvang}",

year = "2012",

month = mar,

doi = "10.1016/j.prostaglandins.2012.01.002",

language = "English",

volume = "97",

pages = "103--108",

journal = "Prostaglandins & Other Lipid Mediators",

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TY - JOUR

T1 - Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

AU - Johansen, Louise Kruse

AU - Iburg, Tine Moesgaard

AU - Nielsen, Ole Lerberg

AU - Leifsson, Páll S.

AU - Dahl-Pedersen, Kirstin

AU - Koch, Janne

AU - Frees, Dorte

AU - Aalbæk, Bent

AU - Heegaard, Peter M. H.

AU - Jensen, Henrik Michael Elvang

PY - 2012/3

Y1 - 2012/3

N2 - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6 h, 12 h, 24 h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.

AB - It is suggested that cyclooxygenase 2 (COX-2) derived prostaglandins contributes to the progressive bone loss seen in osteomyelitis lesions. In the present study we examined the expression of COX-2 in bones from 23 pigs with experimental osteomyelitis. Osteomyelitis was induced with Staphylococcus aureus and groups of animals were euthanized following 6 h, 12 h, 24 h, 2 days, 5 days, 11 days and 15 days, respectively. Expression of COX-2 was evaluated immunohistochemically and combined with characterization of morphological changes in bone tissue. Furthermore, the serum concentrations of alkaline phosphatase and haptoglobin were measured. Extensive COX-2 expression by osteoblasts was present 2 days after inoculation together with many activated osteoclasts. Simultaneously, the serum concentration of alkaline phosphatase decreased whereas the haptoglobin concentration increased. This is the first in vivo study showing an early wave of COX-2 mediated bone resorption during osteomyelitis. Therefore, treatment aiming to reduce the break down of bone tissue directed by the COX-2 pathway might be suggested early in the course of the disease.

U2 - 10.1016/j.prostaglandins.2012.01.002

DO - 10.1016/j.prostaglandins.2012.01.002

M3 - Journal article

C2 - 22266364

SN - 1098-8823

VL - 97

SP - 103

EP - 108

JO - Prostaglandins & Other Lipid Mediators

JF - Prostaglandins & Other Lipid Mediators

IS - 3-4

ER -

Local osteogenic expression of cyclooxygenase-2 and systemic response in porcine models of osteomyelitis

Abstract

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