Live cell microscopy of DNA damage response in Saccharomyces cerevisiae

Sonia Cristina Pinela da Silva; Irene Gallina; Nadine Valerie Eckert-Boulet; Michael Lisby

doi:10.1007/978-1-61779-998-3_30

Live cell microscopy of DNA damage response in Saccharomyces cerevisiae

Sonia Cristina Pinela da Silva, Irene Gallina, Nadine Valerie Eckert-Boulet, Michael Lisby

17 Citations (Scopus)

Abstract

Fluorescence microscopy of the DNA damage response in living cells stands out from many other DNA repair assays by its ability to monitor the response to individual DNA lesions in single cells. This is particularly true in yeast, where the frequency of spontaneous DNA lesions is relatively low compared to organisms with much larger genomes such as mammalian cells. Single cell analysis of individual DNA lesions allows specific events in the DNA damage response to be correlated with cell morphology, cell cycle phase, and other specific characteristics of a particular cell. Moreover, fluorescence live cell imaging allows for multiple cellular markers to be monitored over several hours. This chapter reviews useful fluorescent markers and genotoxic agents for studying the DNA damage response in living cells and provides protocols for live cell imaging, time-lapse microscopy, and for induction of site-specific DNA lesions.

Original language	English
Title of host publication	DNA repair protocols
Number of pages	11
Publisher	Springer Science+Business Media
Publication date	2012
Pages	433-443
ISBN (Print)	978-1-61779-997-6
DOIs	https://doi.org/10.1007/978-1-61779-998-3_30
Publication status	Published - 2012

Series	Methods in Molecular Biology
Volume	920
ISSN	1064-3745

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10.1007/978-1-61779-998-3_30

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Live cell microscopy of DNA damage response in Saccharomyces cerevisiae. / Pinela da Silva, Sonia Cristina; Gallina, Irene; Eckert-Boulet, Nadine Valerie et al.
DNA repair protocols. Springer Science+Business Media, 2012. p. 433-443 (Methods in Molecular Biology, Vol. 920).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Live cell microscopy of DNA damage response in Saccharomyces cerevisiae

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