Introduction of an single nucleodite polymorphism-based "Major Y-chromosome haplogroup typing kit" suitable for predicting the geographical origin of male lineages

TY - JOUR

T1 - Introduction of an single nucleodite polymorphism-based "Major Y-chromosome haplogroup typing kit" suitable for predicting the geographical origin of male lineages

AU - Brión, María

AU - Sanchez, Juan J

AU - Balogh, Kinga

AU - Thacker, Catherine

AU - Blanco-Verea, Alejandro

AU - Børsting, Claus

AU - Stradmann-Bellinghausen, Beate

AU - Bogus, Magdalena

AU - Syndercombe-Court, Denise

AU - Schneider, Peter M

AU - Carracedo, Angel

AU - Morling, Niels

N1 - Keywords: Africa; Asia; Chromosomes, Human, Y; DNA Primers; Europe; Female; Genetics, Population; Haplotypes; Humans; Male; Phylogeny; Polymorphism, Single Nucleotide; South America

PY - 2005

Y1 - 2005

N2 - The European Consortium "High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons--SNPforID", has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A "Major Y-chromosome haplogroup typing kit" has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.

AB - The European Consortium "High-throughput analysis of single nucleotide polymorphisms for the forensic identification of persons--SNPforID", has performed a selection of candidate Y-chromosome single nucleotide polymorphisms (SNPs) for making inferences on the geographic origin of an unknown sample. From more than 200 SNPs compiled in the phylogenetic tree published by the Y-Chromosome Consortium, and looking at the population studies previously published, a package of 29 SNPs has been selected for the identification of major population haplogroups. A "Major Y-chromosome haplogroup typing kit" has been developed, which allows the multiplex amplification of all 29 SNPs in a single reaction. Allele genotyping was performed with a single base extension reaction (minisequencing) detected by CE. The validation of the multiplex was performed in a total of 1126 unrelated males distributed among 12 worldwide populations. The approach takes advantage of the specific geographic distribution of the Y-chromosome haplogroups and demonstrates the utility of binary polymorphisms to infer the origin of a male lineage.

U2 - 10.1002/elps.200500293

DO - 10.1002/elps.200500293

M3 - Journal article

C2 - 16273584

SN - 0173-0835

VL - 26

SP - 4411

EP - 4420

JO - Electrophoresis

JF - Electrophoresis

IS - 23

ER -