In vitro production of bovine embryos: Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

Gianluca Mazzoni; Eduardo Razza; Hanne S. Pedersen; Jan Secher; Haja N. Kadarmideen; Henrik Callesen; Lotte Stroebech; Kristine Freude; Poul Hyttel

doi:10.21451/1984-3143-ar990

In vitro production of bovine embryos: Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

Gianluca Mazzoni, Eduardo Razza, Hanne S. Pedersen, Jan Secher, Haja N. Kadarmideen, Henrik Callesen, Lotte Stroebech, Kristine Freude, Poul Hyttel^*

^*Corresponding author for this work

2 Citations (Scopus)

Abstract

In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and relate these finding to previous data on oocyte developmental competence and ultrastructure. The cumulus/granulosa cell gene expression patterns indicate that early follicular atresia is associated with increased blastocyst yield and this hypothesis is supported by previous data on oocyte competence and ultrastructure.

Original language	English
Journal	Animal Reproduction
Volume	14
Issue number	3
Pages (from-to)	482-489
Number of pages	8
ISSN	1806-9614
DOIs	https://doi.org/10.21451/1984-3143-ar990
Publication status	Published - 2017

Keywords

Atresia
Biomarkers
Cattle
Granulosa cells
IVP
Oocyte competence
Transcriptomics

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10.21451/1984-3143-ar990Licence: CC BY

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title = "In vitro production of bovine embryos: Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence",

abstract = "In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and relate these finding to previous data on oocyte developmental competence and ultrastructure. The cumulus/granulosa cell gene expression patterns indicate that early follicular atresia is associated with increased blastocyst yield and this hypothesis is supported by previous data on oocyte competence and ultrastructure.",

keywords = "Atresia, Biomarkers, Cattle, Granulosa cells, IVP, Oocyte competence, Transcriptomics",

author = "Gianluca Mazzoni and Eduardo Razza and Pedersen, {Hanne S.} and Jan Secher and Kadarmideen, {Haja N.} and Henrik Callesen and Lotte Stroebech and Kristine Freude and Poul Hyttel",

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doi = "10.21451/1984-3143-ar990",

language = "English",

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TY - JOUR

T1 - In vitro production of bovine embryos

T2 - Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

AU - Mazzoni, Gianluca

AU - Razza, Eduardo

AU - Pedersen, Hanne S.

AU - Secher, Jan

AU - Kadarmideen, Haja N.

AU - Callesen, Henrik

AU - Stroebech, Lotte

AU - Freude, Kristine

AU - Hyttel, Poul

PY - 2017

Y1 - 2017

N2 - In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and relate these finding to previous data on oocyte developmental competence and ultrastructure. The cumulus/granulosa cell gene expression patterns indicate that early follicular atresia is associated with increased blastocyst yield and this hypothesis is supported by previous data on oocyte competence and ultrastructure.

AB - In vitro production (IVP) of bovine embryos has become widespread technology implemented in cattle breeding and production. Here, we review novel data on cumulus/granulosa cell gene expression, as determined by RNAseq on cellular material from pooled follicular fluids at the single animal level, and relate these finding to previous data on oocyte developmental competence and ultrastructure. The cumulus/granulosa cell gene expression patterns indicate that early follicular atresia is associated with increased blastocyst yield and this hypothesis is supported by previous data on oocyte competence and ultrastructure.

KW - Atresia

KW - Biomarkers

KW - Cattle

KW - Granulosa cells

KW - IVP

KW - Oocyte competence

KW - Transcriptomics

U2 - 10.21451/1984-3143-ar990

DO - 10.21451/1984-3143-ar990

M3 - Journal article

AN - SCOPUS:85044511925

SN - 1806-9614

VL - 14

SP - 482

EP - 489

JO - Animal Reproduction

JF - Animal Reproduction

IS - 3

ER -

In vitro production of bovine embryos: Cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

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Keywords

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