Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

Rasmus Mortensen; Thomas Nørrelykke Nissen; Sine Fredslund; Ida Rosenkrands; Jan Pravsgaard Christensen; Peter Andersen; Jes Dietrich

doi:10.1038/srep22030

Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

Rasmus Mortensen, Thomas Nørrelykke Nissen, Sine Fredslund, Ida Rosenkrands, Jan Pravsgaard Christensen, Peter Andersen, Jes Dietrich

6 Citations (Scopus)

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Abstract

No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

Original language	English
Article number	22030
Journal	Scientific Reports
Volume	6
Number of pages	11
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep22030
Publication status	Published - 25 Feb 2016

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Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and childrenFinal published version, 1.23 MBLicence: CC BY

Cite this

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title = "Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children",

abstract = "No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.",

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T1 - Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

AU - Mortensen, Rasmus

AU - Nissen, Thomas Nørrelykke

AU - Fredslund, Sine

AU - Rosenkrands, Ida

AU - Christensen, Jan Pravsgaard

AU - Andersen, Peter

AU - Dietrich, Jes

PY - 2016/2/25

Y1 - 2016/2/25

N2 - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

AB - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

U2 - 10.1038/srep22030

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Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

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