Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

TY - JOUR

T1 - Identifying protective Streptococcus pyogenes vaccine antigens recognized by both B and T cells in human adults and children

AU - Mortensen, Rasmus

AU - Nissen, Thomas Nørrelykke

AU - Fredslund, Sine

AU - Rosenkrands, Ida

AU - Christensen, Jan Pravsgaard

AU - Andersen, Peter

AU - Dietrich, Jes

PY - 2016/2/25

Y1 - 2016/2/25

N2 - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

AB - No commercial vaccine exists against Group A streptococci (GAS; Streptococcus pyogenes) and only little is known about anti-GAS protective immunity. In our effort to discover new protective vaccine candidates, we selected 21 antigens based on an in silico evaluation. These were all well-conserved among different GAS strains, upregulated in host-pathogen interaction studies, and predicted to be extracellular or associated with the surface of the bacteria. The antigens were tested for both antibody recognition and T cell responses in human adults and children. The antigenicity of a selected group of antigens was further validated using a high-density peptide array technology that also identified the linear epitopes. Based on immunological recognition, four targets were selected and tested for protective capabilities in an experimental GAS infection model in mice. Shown for the first time, three of these targets (spy0469, spy1228 and spy1801) conferred significant protection whereas one (spy1643) did not.

U2 - 10.1038/srep22030

DO - 10.1038/srep22030

M3 - Journal article

C2 - 26911649

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 22030

ER -