High throughput screening of starch structures using carbohydrate microarrays

Vanja Tanackovic; Maja Gro Rydahl; Henriette Lodberg Pedersen; Mohammed Saddik Motawie; Shahnoor Sultana Shaik; Maria Dalgaard Mikkelsen; Susanne Langgård Jensen; Jonatan Ulrik Fangel; William George Tycho Willats; Andreas Blennow

doi:10.1038/srep30551

High throughput screening of starch structures using carbohydrate microarrays

Vanja Tanackovic, Maja Gro Rydahl, Henriette Lodberg Pedersen, Mohammed Saddik Motawie, Shahnoor Sultana Shaik, Maria Dalgaard Mikkelsen, Susanne Langgård Jensen, Jonatan Ulrik Fangel, William George Tycho Willats, Andreas Blennow

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Abstract

In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

Original language	English
Article number	30551
Journal	Scientific Reports
Volume	6
Number of pages	9
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep30551
Publication status	Published - 29 Jul 2016

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Journal Article

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title = "High throughput screening of starch structures using carbohydrate microarrays",

abstract = "In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.",

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AU - Tanackovic, Vanja

AU - Rydahl, Maja Gro

AU - Pedersen, Henriette Lodberg

AU - Motawie, Mohammed Saddik

AU - Shaik, Shahnoor Sultana

AU - Mikkelsen, Maria Dalgaard

AU - Jensen, Susanne Langgård

AU - Fangel, Jonatan Ulrik

AU - Willats, William George Tycho

AU - Blennow, Andreas

PY - 2016/7/29

Y1 - 2016/7/29

N2 - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

AB - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.

KW - Journal Article

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DO - 10.1038/srep30551

M3 - Journal article

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SN - 2045-2322

VL - 6

JO - Scientific Reports

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High throughput screening of starch structures using carbohydrate microarrays

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