TY - JOUR
T1 - High throughput screening of starch structures using carbohydrate microarrays
AU - Tanackovic, Vanja
AU - Rydahl, Maja Gro
AU - Pedersen, Henriette Lodberg
AU - Motawie, Mohammed Saddik
AU - Shaik, Shahnoor Sultana
AU - Mikkelsen, Maria Dalgaard
AU - Jensen, Susanne Langgård
AU - Fangel, Jonatan Ulrik
AU - Willats, William George Tycho
AU - Blennow, Andreas
PY - 2016/7/29
Y1 - 2016/7/29
N2 - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.
AB - In this study we introduce the starch-recognising carbohydrate binding module family 20 (CBM20) from Aspergillus niger for screening biological variations in starch molecular structure using high throughput carbohydrate microarray technology. Defined linear, branched and phosphorylated maltooligosaccharides, pure starch samples including a variety of different structures with variations in the amylopectin branching pattern, amylose content and phosphate content, enzymatically modified starches and glycogen were included. Using this technique, different important structures, including amylose content and branching degrees could be differentiated in a high throughput fashion. The screening method was validated using transgenic barley grain analysed during development and subjected to germination. Typically, extreme branching or linearity were detected less than normal starch structures. The method offers the potential for rapidly analysing resistant and slowly digested dietary starches.
KW - Journal Article
U2 - 10.1038/srep30551
DO - 10.1038/srep30551
M3 - Journal article
C2 - 27468930
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 30551
ER -