Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

A Ramsay; A R Williams; S M Thamsborg; I Mueller-Harvey

doi:10.1016/j.phytochem.2015.12.005

Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

A Ramsay, A R Williams, S M Thamsborg, I Mueller-Harvey

13 Citations (Scopus)

Abstract

Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.

Original language	English
Journal	Phytochemistry
Volume	122
Pages (from-to)	146-153
Number of pages	8
ISSN	0031-9422
DOIs	https://doi.org/10.1016/j.phytochem.2015.12.005
Publication status	Published - 1 Feb 2016

Keywords

Faculty of Health and Medical Sciences
Vitellaria paradoxa
Shea meal
Thiolysis
Proanthocyanidins
Galloylated
Ascaris suum
Anthelmintic
LC–MS

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10.1016/j.phytochem.2015.12.005

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@article{0b65dbaa0e114f518b6a20cac434704e,

title = "Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum",

abstract = "Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.",

keywords = "Faculty of Health and Medical Sciences, Vitellaria paradoxa, Shea meal, Thiolysis, Proanthocyanidins, Galloylated, Ascaris suum, Anthelmintic, LC–MS",

author = "A Ramsay and Williams, {A R} and Thamsborg, {S M} and I Mueller-Harvey",

year = "2016",

month = feb,

day = "1",

doi = "10.1016/j.phytochem.2015.12.005",

language = "English",

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T1 - Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

AU - Ramsay, A

AU - Williams, A R

AU - Thamsborg, S M

AU - Mueller-Harvey, I

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.

AB - Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.

KW - Faculty of Health and Medical Sciences

KW - Vitellaria paradoxa

KW - Shea meal

KW - Thiolysis

KW - Proanthocyanidins

KW - Galloylated

KW - Ascaris suum

KW - Anthelmintic

KW - LC–MS

U2 - 10.1016/j.phytochem.2015.12.005

DO - 10.1016/j.phytochem.2015.12.005

M3 - Journal article

C2 - 26708339

SN - 0031-9422

VL - 122

SP - 146

EP - 153

JO - Phytochemistry

JF - Phytochemistry

ER -

Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

Abstract

Keywords

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