Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

TY - JOUR

T1 - Galloylated proanthocyanidins from shea (Vitellaria paradoxa) meal have potent anthelmintic activity against Ascaris suum

AU - Ramsay, A

AU - Williams, A R

AU - Thamsborg, S M

AU - Mueller-Harvey, I

N1 - Crown Copyright © 2015. Published by Elsevier Ltd. All rights reserved.

PY - 2016/2/1

Y1 - 2016/2/1

N2 - Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.

AB - Proanthocyanidins (PA) from shea (Vitellaria paradoxa) meal were investigated by thiolytic degradation with benzyl mercaptan and the reaction products were analysed by high performance liquid chromatography-mass spectrometry. These PA were galloylated (≈40%), contained only B-type linkages and had a high proportion of prodelphinidins (>70%). The mean degree of polymerisation was 8 (i.e. average molecular size was 2384Da) and epigallocatechin gallate (EGCg) was the major flavan-3-ol subunit in PA. Shea meal also proved to be a potentially valuable source for extracting free flavan-3-ol-O-gallates, especially EGCg (575mg/kg meal), which is known for its health and anti-parasitic benefits. Proanthocyanidins were isolated and tested for bioactivity against Ascaris suum, which is an important parasite of pigs. Migration and motility tests revealed that these PA have potent activity against this parasitic nematode.

KW - Faculty of Health and Medical Sciences

KW - Vitellaria paradoxa

KW - Shea meal

KW - Thiolysis

KW - Proanthocyanidins

KW - Galloylated

KW - Ascaris suum

KW - Anthelmintic

KW - LC–MS

U2 - 10.1016/j.phytochem.2015.12.005

DO - 10.1016/j.phytochem.2015.12.005

M3 - Journal article

C2 - 26708339

SN - 0031-9422

VL - 122

SP - 146

EP - 153

JO - Phytochemistry

JF - Phytochemistry

ER -