Evaluation of plasma and urinary levels of 6-keto-prostaglandin F1a as a marker for asymptomatic myxomatous mitral valve disease in dogs

Caroline Elisabeth Rasmussen; Anna Vilhelmina Sundqvist; Christina Tirsdal Kjempff; Inge Tarnow; Mads Kjelgaard-Hansen; Thea Sigbrit Kamstrup; Annelise Sterup; Tina Møller Sørensen; Lisbeth Høier Olsen

doi:10.1016/j.tvjl.2009.02.012

Evaluation of plasma and urinary levels of 6-keto-prostaglandin F_1a as a marker for asymptomatic myxomatous mitral valve disease in dogs

Caroline Elisabeth Rasmussen, Anna Vilhelmina Sundqvist, Christina Tirsdal Kjempff, Inge Tarnow, Mads Kjelgaard-Hansen, Thea Sigbrit Kamstrup, Annelise Sterup, Tina Møller Sørensen, Lisbeth Høier Olsen

2 Citations (Scopus)

Abstract

Endothelial dysfunction might be involved in the pathogenesis of myxomatous mitral valve disease (MMVD). The aims of this study were (1) to validate an enzyme immunoassay (EIA) for canine 6-keto-prostaglandin (PG)F_1α (prostacyclin metabolite and marker for endothelial function) and (2) to compare plasma and urinary 6-keto-PGF_1α in dogs with asymptomatic MMVD. The study included two breeds predisposed to MMVD and two control groups (Cairn terriers and dogs of different breeds). Echocardiography was used to estimate the severity of MMVD. The intra- and inter-assay coefficients of variation were between 3.1% and 24.5% in the assay range. No echocardiographic parameter was correlated with plasma or urinary 6-keto-PGF_1α (P > 0.05), but all control dogs had lower urinary 6-keto-PGF_1α (P < 0.02) and the Cairn terriers had higher plasma 6-keto-PGF_1α (P < 0.02). The EIA appeared valid for measuring canine 6-keto-PGF_1α in plasma and urine. It is suggested that 6-keto-PGF_1α levels are related to breed and not MMVD in asymptomatic stages.

Original language	English
Journal	Veterinary Journal
Volume	184
Issue number	2
Pages (from-to)	241-246
Number of pages	6
ISSN	1090-0233
DOIs	https://doi.org/10.1016/j.tvjl.2009.02.012
Publication status	Published - May 2010

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Rasmussen, C. E., Sundqvist, A. V., Kjempff, C. T., Tarnow, I., Kjelgaard-Hansen, M., Kamstrup, T. S., Sterup, A., Sørensen, T. M., & Olsen, L. H. (2010). Evaluation of plasma and urinary levels of 6-keto-prostaglandin F_1a as a marker for asymptomatic myxomatous mitral valve disease in dogs. Veterinary Journal, 184(2), 241-246. https://doi.org/10.1016/j.tvjl.2009.02.012

Evaluation of plasma and urinary levels of 6-keto-prostaglandin F_1a as a marker for asymptomatic myxomatous mitral valve disease in dogs. / Rasmussen, Caroline Elisabeth ; Sundqvist, Anna Vilhelmina; Kjempff, Christina Tirsdal et al.

In: Veterinary Journal, Vol. 184, No. 2, 05.2010, p. 241-246.

Research output: Contribution to journal › Journal article › Research › peer-review

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title = "Evaluation of plasma and urinary levels of 6-keto-prostaglandin F1a as a marker for asymptomatic myxomatous mitral valve disease in dogs",

abstract = "Endothelial dysfunction might be involved in the pathogenesis of myxomatous mitral valve disease (MMVD). The aims of this study were (1) to validate an enzyme immunoassay (EIA) for canine 6-keto-prostaglandin (PG)F1α (prostacyclin metabolite and marker for endothelial function) and (2) to compare plasma and urinary 6-keto-PGF1α in dogs with asymptomatic MMVD. The study included two breeds predisposed to MMVD and two control groups (Cairn terriers and dogs of different breeds). Echocardiography was used to estimate the severity of MMVD. The intra- and inter-assay coefficients of variation were between 3.1% and 24.5% in the assay range. No echocardiographic parameter was correlated with plasma or urinary 6-keto-PGF1α (P > 0.05), but all control dogs had lower urinary 6-keto-PGF1α (P < 0.02) and the Cairn terriers had higher plasma 6-keto-PGF1α (P < 0.02). The EIA appeared valid for measuring canine 6-keto-PGF1α in plasma and urine. It is suggested that 6-keto-PGF1α levels are related to breed and not MMVD in asymptomatic stages.",

author = "Rasmussen, {Caroline Elisabeth} and Sundqvist, {Anna Vilhelmina} and Kjempff, {Christina Tirsdal} and Inge Tarnow and Mads Kjelgaard-Hansen and Kamstrup, {Thea Sigbrit} and Annelise Sterup and S{\o}rensen, {Tina M{\o}ller} and Olsen, {Lisbeth H{\o}ier}",

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AU - Rasmussen, Caroline Elisabeth

AU - Sundqvist, Anna Vilhelmina

AU - Kjempff, Christina Tirsdal

AU - Tarnow, Inge

AU - Kjelgaard-Hansen, Mads

AU - Kamstrup, Thea Sigbrit

AU - Sterup, Annelise

AU - Sørensen, Tina Møller

AU - Olsen, Lisbeth Høier

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N2 - Endothelial dysfunction might be involved in the pathogenesis of myxomatous mitral valve disease (MMVD). The aims of this study were (1) to validate an enzyme immunoassay (EIA) for canine 6-keto-prostaglandin (PG)F1α (prostacyclin metabolite and marker for endothelial function) and (2) to compare plasma and urinary 6-keto-PGF1α in dogs with asymptomatic MMVD. The study included two breeds predisposed to MMVD and two control groups (Cairn terriers and dogs of different breeds). Echocardiography was used to estimate the severity of MMVD. The intra- and inter-assay coefficients of variation were between 3.1% and 24.5% in the assay range. No echocardiographic parameter was correlated with plasma or urinary 6-keto-PGF1α (P > 0.05), but all control dogs had lower urinary 6-keto-PGF1α (P < 0.02) and the Cairn terriers had higher plasma 6-keto-PGF1α (P < 0.02). The EIA appeared valid for measuring canine 6-keto-PGF1α in plasma and urine. It is suggested that 6-keto-PGF1α levels are related to breed and not MMVD in asymptomatic stages.

AB - Endothelial dysfunction might be involved in the pathogenesis of myxomatous mitral valve disease (MMVD). The aims of this study were (1) to validate an enzyme immunoassay (EIA) for canine 6-keto-prostaglandin (PG)F1α (prostacyclin metabolite and marker for endothelial function) and (2) to compare plasma and urinary 6-keto-PGF1α in dogs with asymptomatic MMVD. The study included two breeds predisposed to MMVD and two control groups (Cairn terriers and dogs of different breeds). Echocardiography was used to estimate the severity of MMVD. The intra- and inter-assay coefficients of variation were between 3.1% and 24.5% in the assay range. No echocardiographic parameter was correlated with plasma or urinary 6-keto-PGF1α (P > 0.05), but all control dogs had lower urinary 6-keto-PGF1α (P < 0.02) and the Cairn terriers had higher plasma 6-keto-PGF1α (P < 0.02). The EIA appeared valid for measuring canine 6-keto-PGF1α in plasma and urine. It is suggested that 6-keto-PGF1α levels are related to breed and not MMVD in asymptomatic stages.

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ER -

Evaluation of plasma and urinary levels of 6-keto-prostaglandin F1a as a marker for asymptomatic myxomatous mitral valve disease in dogs

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Evaluation of plasma and urinary levels of 6-keto-prostaglandin F_1a as a marker for asymptomatic myxomatous mitral valve disease in dogs