Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells

T E Maar; Trine Meldgaard Lund; G Gegelashvili; R Hartmann-Petersen; Michael J. Moran; H Pasantes-Morales; V Berezin; E Bock; A Schousboe

doi:10.1007/BF01345281

Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells

T E Maar, Trine Meldgaard Lund, G Gegelashvili, R Hartmann-Petersen, Michael J. Moran, H Pasantes-Morales, V Berezin, E Bock, A Schousboe

6 Citations (Scopus)

Abstract

Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.

Original language	English
Journal	Amino Acids
Volume	15
Issue number	1
Pages (from-to)	77-88
Number of pages	12
ISSN	0939-4451
DOIs	https://doi.org/10.1007/BF01345281
Publication status	Published - 1998

Keywords

Alternative Splicing
Animals
Biological Transport
Cell Movement
Cells, Cultured
Cerebellum
Gene Expression
Mice
Nerve Net
Neural Cell Adhesion Molecules
Neurons
RNA, Messenger
Taurine

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10.1007/BF01345281

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@article{d42c197074c911dbbee902004c4f4f50,

title = "Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells",

abstract = "Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.",

keywords = "Alternative Splicing, Animals, Biological Transport, Cell Movement, Cells, Cultured, Cerebellum, Gene Expression, Mice, Nerve Net, Neural Cell Adhesion Molecules, Neurons, RNA, Messenger, Taurine",

author = "Maar, {T E} and Lund, {Trine Meldgaard} and G Gegelashvili and R Hartmann-Petersen and Moran, {Michael J.} and H Pasantes-Morales and V Berezin and E Bock and A Schousboe",

year = "1998",

doi = "10.1007/BF01345281",

language = "English",

volume = "15",

pages = "77--88",

journal = "Amino Acids",

issn = "0939-4451",

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T1 - Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells

AU - Maar, T E

AU - Lund, Trine Meldgaard

AU - Gegelashvili, G

AU - Hartmann-Petersen, R

AU - Moran, Michael J.

AU - Pasantes-Morales, H

AU - Berezin, V

AU - Bock, E

AU - Schousboe, A

PY - 1998

Y1 - 1998

N2 - Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.

AB - Cultures of dissociated cerebellum from 5- to 6-day-old mice as well as of the N2A neuronal cell line were exposed to guanidino ethane sulfonate (GES, 2-5 mM) to reduce the cellular taurine content. Control cultures were kept in culture medium or medium containing 2-5 mM GES plus 2-5 mM taurine to restore the intracellular taurine content. Taurine depletion led to changes in the expression of certain splice variants of NCAM mRNA such as the AAG and the VASE containing forms, while no differences were seen in the expression of the three forms of NCAM protein. In the N2A cells taurine depletion led to a decreased migration rate of the cells. The results suggest that the reduced migration rate of neurons caused by taurine depletion may be correlated to changes in expression of certain adhesion molecules such as NCAM. Moreover, taurine appears to be involved in regulation of transcription processes.

KW - Alternative Splicing

KW - Animals

KW - Biological Transport

KW - Cell Movement

KW - Cells, Cultured

KW - Cerebellum

KW - Gene Expression

KW - Mice

KW - Nerve Net

KW - Neural Cell Adhesion Molecules

KW - Neurons

KW - RNA, Messenger

KW - Taurine

U2 - 10.1007/BF01345281

DO - 10.1007/BF01345281

M3 - Journal article

C2 - 9871488

SN - 0939-4451

VL - 15

SP - 77

EP - 88

JO - Amino Acids

JF - Amino Acids

IS - 1

ER -

Effects of taurine depletion on cell migration and NCAM expression in cultures of dissociated mouse cerebellum and N2A cells

Abstract

Keywords

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