DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

C Lukas; J Bartkova; L Latella; J Falck; Niels Mailand; T Schroeder; M Sehested; J Lukas; J Bartek

DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

C Lukas, J Bartkova, L Latella, J Falck, Niels Mailand, T Schroeder, M Sehested, J Lukas, J Bartek

106 Citations (Scopus)

Abstract

The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

Original language	English
Journal	Cancer Research
Volume	61
Issue number	13
Pages (from-to)	4990-3
Number of pages	4
ISSN	0008-5472
Publication status	Published - 1 Jul 2001
Externally published	Yes

Keywords

Antibodies, Monoclonal
Cell Cycle
Cell Differentiation
Cell Division
Cell Line
Checkpoint Kinase 2
DNA Damage
Enzyme Activation
Fibroblasts
G1 Phase
Humans
Osteosarcoma
Protein Kinases
Protein-Serine-Threonine Kinases
S Phase
Tumor Cells, Cultured

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http://cancerres.aacrjournals.org/content/61/13/4990.full

Cite this

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title = "DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology",

abstract = "The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.",

keywords = "Antibodies, Monoclonal, Cell Cycle, Cell Differentiation, Cell Division, Cell Line, Checkpoint Kinase 2, DNA Damage, Enzyme Activation, Fibroblasts, G1 Phase, Humans, Osteosarcoma, Protein Kinases, Protein-Serine-Threonine Kinases, S Phase, Tumor Cells, Cultured",

author = "C Lukas and J Bartkova and L Latella and J Falck and Niels Mailand and T Schroeder and M Sehested and J Lukas and J Bartek",

year = "2001",

month = jul,

day = "1",

language = "English",

volume = "61",

pages = "4990--3",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research",

number = "13",

}

TY - JOUR

T1 - DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

AU - Lukas, C

AU - Bartkova, J

AU - Latella, L

AU - Falck, J

AU - Mailand, Niels

AU - Schroeder, T

AU - Sehested, M

AU - Lukas, J

AU - Bartek, J

PY - 2001/7/1

Y1 - 2001/7/1

N2 - The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

AB - The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

KW - Antibodies, Monoclonal

KW - Cell Cycle

KW - Cell Differentiation

KW - Cell Division

KW - Cell Line

KW - Checkpoint Kinase 2

KW - DNA Damage

KW - Enzyme Activation

KW - Fibroblasts

KW - G1 Phase

KW - Humans

KW - Osteosarcoma

KW - Protein Kinases

KW - Protein-Serine-Threonine Kinases

KW - S Phase

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 11431331

SN - 0008-5472

VL - 61

SP - 4990

EP - 4993

JO - Cancer Research

JF - Cancer Research

IS - 13

ER -

DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

Abstract

Keywords

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