DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

C Lukas; J Bartkova; L Latella; J Falck; Niels Mailand; T Schroeder; M Sehested; J Lukas; J Bartek

DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

C Lukas, J Bartkova, L Latella, J Falck, Niels Mailand, T Schroeder, M Sehested, J Lukas, J Bartek

106 Citationer (Scopus)

Abstract

The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

Originalsprog	Engelsk
Tidsskrift	Cancer Research
Vol/bind	61
Udgave nummer	13
Sider (fra-til)	4990-3
Antal sider	4
ISSN	0008-5472
Status	Udgivet - 1 jul. 2001
Udgivet eksternt	Ja

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http://cancerres.aacrjournals.org/content/61/13/4990.full

Citationsformater

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title = "DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology",

abstract = "The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.",

keywords = "Antibodies, Monoclonal, Cell Cycle, Cell Differentiation, Cell Division, Cell Line, Checkpoint Kinase 2, DNA Damage, Enzyme Activation, Fibroblasts, G1 Phase, Humans, Osteosarcoma, Protein Kinases, Protein-Serine-Threonine Kinases, S Phase, Tumor Cells, Cultured",

author = "C Lukas and J Bartkova and L Latella and J Falck and Niels Mailand and T Schroeder and M Sehested and J Lukas and J Bartek",

year = "2001",

month = jul,

day = "1",

language = "English",

volume = "61",

pages = "4990--3",

journal = "Cancer Research",

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TY - JOUR

T1 - DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

AU - Lukas, C

AU - Bartkova, J

AU - Latella, L

AU - Falck, J

AU - Mailand, Niels

AU - Schroeder, T

AU - Sehested, M

AU - Lukas, J

AU - Bartek, J

PY - 2001/7/1

Y1 - 2001/7/1

N2 - The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

AB - The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase restricted to S-G(2) phases, Chk2 remains activatable even in quiescent and differentiating cells. In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or cytoplasmic in static tissues such as muscle or brain. These data highlight striking differences between Chk2 and Chk1 and show unexpected correlation of Chk2 expression with tissue biology.

KW - Antibodies, Monoclonal

KW - Cell Cycle

KW - Cell Differentiation

KW - Cell Division

KW - Cell Line

KW - Checkpoint Kinase 2

KW - DNA Damage

KW - Enzyme Activation

KW - Fibroblasts

KW - G1 Phase

KW - Humans

KW - Osteosarcoma

KW - Protein Kinases

KW - Protein-Serine-Threonine Kinases

KW - S Phase

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 11431331

SN - 0008-5472

VL - 61

SP - 4990

EP - 4993

JO - Cancer Research

JF - Cancer Research

IS - 13

ER -

DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology

Abstract

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