DNA conformational analysis in solution by uranyl mediated photocleavage

Peter E. Nielsen; N E Møllegaard; C Jeppesen

DNA conformational analysis in solution by uranyl mediated photocleavage

Peter E. Nielsen, N E Møllegaard, C Jeppesen

Department of Cellular and Molecular Medicine

37 Citations (Scopus)

Abstract

Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.

Original language	English
Journal	Nucleic Acids Research
Volume	18
Issue number	13
Pages (from-to)	3847-51
Number of pages	4
ISSN	0305-1048
Publication status	Published - 1990

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@article{15bed7c0e60011ddbf70000ea68e967b,

title = "DNA conformational analysis in solution by uranyl mediated photocleavage",

abstract = "Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.",

author = "Nielsen, {Peter E.} and M{\o}llegaard, {N E} and C Jeppesen",

note = "Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus",

year = "1990",

language = "English",

volume = "18",

pages = "3847--51",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "13",

}

TY - JOUR

T1 - DNA conformational analysis in solution by uranyl mediated photocleavage

AU - Nielsen, Peter E.

AU - Møllegaard, N E

AU - Jeppesen, C

N1 - Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus

PY - 1990

Y1 - 1990

N2 - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.

AB - Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.

M3 - Journal article

C2 - 2374711

SN - 0305-1048

VL - 18

SP - 3847

EP - 3851

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 13

ER -

DNA conformational analysis in solution by uranyl mediated photocleavage

Abstract

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