@article{15bed7c0e60011ddbf70000ea68e967b,
title = "DNA conformational analysis in solution by uranyl mediated photocleavage",
abstract = "Uranyl mediated photocleavage of double stranded DNA is proposed as a general probing for DNA helix conformation in terms of minor groove width/electronegative potential. Specifically, it is found that A/T-tracts known to constitute strong distamycin binding sites are preferentially photocleaved by uranyl in a way indicating strongest uranyl binding at the center of the minor groove of the AT-region. The A-tracts of kinetoplast DNA show the highest reactivity at the 3'-end of the tract--as opposed to cleavage by EDTA/Fell--in accordance with the minor groove being more narrow at this end. Finally, uranyl photocleavage of the internal control region (ICR) of the 5S-RNA gene yields a cleavage modulation pattern fully compatible with that obtained by DNase I which also--in a more complex way--senses DNA minor groove width.",
author = "Nielsen, {Peter E.} and M{\o}llegaard, {N E} and C Jeppesen",
note = "Keywords: Animals; Base Sequence; DNA; Densitometry; Molecular Sequence Data; Nucleic Acid Conformation; Photochemistry; Uranium; Uranyl Nitrate; Xenopus",
year = "1990",
language = "English",
volume = "18",
pages = "3847--51",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "13",
}