Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs

    5 Citations (Scopus)

    Abstract

    AIM: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.

    MATERIALS & METHODS: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.

    RESULTS: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).

    CONCLUSION: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.

    Original languageEnglish
    JournalEpigenomics
    Volume10
    Issue number4
    Pages (from-to)395-408
    Number of pages14
    ISSN1750-1911
    DOIs
    Publication statusPublished - 1 Apr 2018

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