Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs

TY - JOUR

T1 - Diet-dependent changes in the intestinal DNA methylome after introduction of enteral feeding in preterm pigs

AU - Pan, Xiaoyu

AU - Gong, Desheng

AU - Gao, Fei

AU - Sangild, Per Torp

PY - 2018/4/1

Y1 - 2018/4/1

N2 - AIM: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.MATERIALS & METHODS: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.RESULTS: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).CONCLUSION: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.

AB - AIM: To examine how enteral feeding affects the intestinal epigenome and gene expression just after preterm birth.MATERIALS & METHODS: Intestinal tissue from preterm pigs, modeling preterm infants, was collected at birth and 5 days after gradual introduction of infant formula or bovine colostrum. The intestinal tissue was analyzed by reduced representation bisulfite sequencing and real-time qPCR.RESULTS: Relative to colostrum, formula increased bacterial epithelial adherence and lipopolysaccharide binding protein (LBP) expression, which was regulated by promoter methylation. Diet-dependent changes in DNA methylation and/or mRNA expression were related to innate immune response, hypoxia, angiogenesis and epithelial-mesenchymal transition pathways (e.g., TTC38, IL8, C3, HIF1A and VEGFR1).CONCLUSION: Epigenetic changes may mediate important effects of the first feeding on intestinal development in preterm neonates.

U2 - 10.2217/epi-2017-0122

DO - 10.2217/epi-2017-0122

M3 - Journal article

C2 - 29587528

SN - 1750-1911

VL - 10

SP - 395

EP - 408

JO - Epigenomics

JF - Epigenomics

IS - 4

ER -