Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning

Morten Egevang Jørgensen; Nikolai Wulff; Majse Nafisi; Deyang Xu; Cuiwei Wang; Sophie Konstanze Lambertz; Zeinu Mussa Belew; Hussam Hassan Nour-Eldin

doi:10.21769/BioProtoc.2615

Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USER^TM cloning

Morten Egevang Jørgensen, Nikolai Wulff, Majse Nafisi, Deyang Xu, Cuiwei Wang, Sophie Konstanze Lambertz, Zeinu Mussa Belew, Hussam Hassan Nour-Eldin

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Abstract

This protocol describes how to order and directly assemble uracil-containing non-clonal DNA
fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of
making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is
highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from
any synthesizing company. The protocol drastically reduces time and handling between receiving the
synthesized DNA fragments and transforming with vector and DNA fragment(s).

Original language	English
Article number	e2615
Journal	Bio-protocol
Volume	7
Issue number	22
Number of pages	11
ISSN	2331-8325
DOIs	https://doi.org/10.21769/BioProtoc.2615
Publication status	Published - 2017

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10.21769/BioProtoc.2615Licence: CC BY

Bio-protocol2615Final published version, 2.82 MBLicence: CC BY

Cite this

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title = "Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning",

abstract = "This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).",

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AU - Jørgensen, Morten Egevang

AU - Wulff, Nikolai

AU - Nafisi, Majse

AU - Xu, Deyang

AU - Wang, Cuiwei

AU - Lambertz, Sophie Konstanze

AU - Belew, Zeinu Mussa

AU - Nour-Eldin, Hussam Hassan

PY - 2017

Y1 - 2017

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AB - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).

U2 - 10.21769/BioProtoc.2615

DO - 10.21769/BioProtoc.2615

M3 - Journal article

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