Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning

Morten Egevang Jørgensen; Nikolai Wulff; Majse Nafisi; Deyang Xu; Cuiwei Wang; Sophie Konstanze Lambertz; Zeinu Mussa Belew; Hussam Hassan Nour-Eldin

doi:10.21769/BioProtoc.2615

Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USER^TM cloning

Morten Egevang Jørgensen, Nikolai Wulff, Majse Nafisi, Deyang Xu, Cuiwei Wang, Sophie Konstanze Lambertz, Zeinu Mussa Belew, Hussam Hassan Nour-Eldin

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Abstract

This protocol describes how to order and directly assemble uracil-containing non-clonal DNA
fragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal of
making synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol is
highly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained from
any synthesizing company. The protocol drastically reduces time and handling between receiving the
synthesized DNA fragments and transforming with vector and DNA fragment(s).

Originalsprog	Engelsk
Artikelnummer	e2615
Tidsskrift	Bio-protocol
Vol/bind	7
Udgave nummer	22
Antal sider	11
ISSN	2331-8325
DOI	https://doi.org/10.21769/BioProtoc.2615
Status	Udgivet - 2017

Adgang til dokumentet

10.21769/BioProtoc.2615Licens: CC BY

Bio-protocol2615Forlagets udgivne version, 2,82 MBLicens: CC BY

Citationsformater

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abstract = "This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).",

author = "J{\o}rgensen, {Morten Egevang} and Nikolai Wulff and Majse Nafisi and Deyang Xu and Cuiwei Wang and Lambertz, {Sophie Konstanze} and Belew, {Zeinu Mussa} and Nour-Eldin, {Hussam Hassan}",

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AU - Jørgensen, Morten Egevang

AU - Wulff, Nikolai

AU - Nafisi, Majse

AU - Xu, Deyang

AU - Wang, Cuiwei

AU - Lambertz, Sophie Konstanze

AU - Belew, Zeinu Mussa

AU - Nour-Eldin, Hussam Hassan

PY - 2017

Y1 - 2017

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AB - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).

U2 - 10.21769/BioProtoc.2615

DO - 10.21769/BioProtoc.2615

M3 - Journal article

SN - 2331-8325

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