Comparative fermentation of insoluble carbohydrates in an in vitro human feces model spiked with Lactobacillus acidophilus NCFM

Anne Knudsen; Gabriella Christina van Zanten; Susanne Langgård Jensen; Sofia D. Forssten; Markku Saarinen; Sampo J. Lahtinen; Ole Bandsholm; Birte Svensson; Lene Jespersen; Andreas Blennow

doi:10.1002/star.201200091

Comparative fermentation of insoluble carbohydrates in an in vitro human feces model spiked with Lactobacillus acidophilus NCFM

Anne Knudsen, Gabriella Christina van Zanten, Susanne Langgård Jensen, Sofia D. Forssten, Markku Saarinen, Sampo J. Lahtinen, Ole Bandsholm, Birte Svensson, Lene Jespersen, Andreas Blennow

6 Citations (Scopus)

Abstract

Dietary fiber and slow carbohydrates can have differential beneficial effects on gut microbial composition and metabolism. Insoluble substrates such as RS cannot be used in continuous simulation systems and we tested an in vitro batch fermentation system for possible prebiotic effects using waxy maize starch granules, pectin-rich potato fiber, and potato lintner starch tested with human fresh fecal microbiota spiked with the probiotic Lactobacillus acidophilus NCFM. Microbial quantification by real-time polymerase chain-reaction (qRT-PCR) revealed that Bacteroidetes was specifically suppressed by each insoluble carbohydrate resulting in a clear decrease in the ratio of Bacteroidetes and Firmicutes. Notably, all carbohydrates tested appeared to block the formation of the potentially harmful branched chain fatty acids (BCFA) fermentation products, but supported lactobacilli growth and increase in lactic acid production. Potato lintner starch had the greatest effect. Insoluble carbohydrates also suppressed production of SCFAs as compared to the control medium. Importantly, potato lintner starch most efficiently suppressed the ratio between the Bacteroidetes and Firmicutes and suppressed growth of bifidobacteria, Enterobactericeae and Faecalibacterium prausnizii. Degradative resistance of the lintner starch were linked to these effects. Problems using continuous fermentation systems for insoluble prebiotics and interpretation of batch fermentation data are discussed.

Original language	English
Journal	Starch
Volume	65
Issue number	3-4
Pages (from-to)	346-353
Number of pages	8
ISSN	0038-9056
DOIs	https://doi.org/10.1002/star.201200091
Publication status	Published - Mar 2013

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@article{9a13b213a3cc436f88238adc7a6e2528,

title = "Comparative fermentation of insoluble carbohydrates in an in vitro human feces model spiked with Lactobacillus acidophilus NCFM",

abstract = "Dietary fiber and slow carbohydrates can have differential beneficial effects on gut microbial composition and metabolism. Insoluble substrates such as RS cannot be used in continuous simulation systems and we tested an in vitro batch fermentation system for possible prebiotic effects using waxy maize starch granules, pectin-rich potato fiber, and potato lintner starch tested with human fresh fecal microbiota spiked with the probiotic Lactobacillus acidophilus NCFM. Microbial quantification by real-time polymerase chain-reaction (qRT-PCR) revealed that Bacteroidetes was specifically suppressed by each insoluble carbohydrate resulting in a clear decrease in the ratio of Bacteroidetes and Firmicutes. Notably, all carbohydrates tested appeared to block the formation of the potentially harmful branched chain fatty acids (BCFA) fermentation products, but supported lactobacilli growth and increase in lactic acid production. Potato lintner starch had the greatest effect. Insoluble carbohydrates also suppressed production of SCFAs as compared to the control medium. Importantly, potato lintner starch most efficiently suppressed the ratio between the Bacteroidetes and Firmicutes and suppressed growth of bifidobacteria, Enterobactericeae and Faecalibacterium prausnizii. Degradative resistance of the lintner starch were linked to these effects. Problems using continuous fermentation systems for insoluble prebiotics and interpretation of batch fermentation data are discussed.",

author = "Anne Knudsen and {van Zanten}, {Gabriella Christina} and Jensen, {Susanne Langg{\aa}rd} and Forssten, {Sofia D.} and Markku Saarinen and Lahtinen, {Sampo J.} and Ole Bandsholm and Birte Svensson and Lene Jespersen and Andreas Blennow",

year = "2013",

month = mar,

doi = "10.1002/star.201200091",

language = "English",

volume = "65",

pages = "346--353",

journal = "Starch",

issn = "0038-9056",

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number = "3-4",

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TY - JOUR

T1 - Comparative fermentation of insoluble carbohydrates in an in vitro human feces model spiked with Lactobacillus acidophilus NCFM

AU - Knudsen, Anne

AU - van Zanten, Gabriella Christina

AU - Jensen, Susanne Langgård

AU - Forssten, Sofia D.

AU - Saarinen, Markku

AU - Lahtinen, Sampo J.

AU - Bandsholm, Ole

AU - Svensson, Birte

AU - Jespersen, Lene

AU - Blennow, Andreas

PY - 2013/3

Y1 - 2013/3

N2 - Dietary fiber and slow carbohydrates can have differential beneficial effects on gut microbial composition and metabolism. Insoluble substrates such as RS cannot be used in continuous simulation systems and we tested an in vitro batch fermentation system for possible prebiotic effects using waxy maize starch granules, pectin-rich potato fiber, and potato lintner starch tested with human fresh fecal microbiota spiked with the probiotic Lactobacillus acidophilus NCFM. Microbial quantification by real-time polymerase chain-reaction (qRT-PCR) revealed that Bacteroidetes was specifically suppressed by each insoluble carbohydrate resulting in a clear decrease in the ratio of Bacteroidetes and Firmicutes. Notably, all carbohydrates tested appeared to block the formation of the potentially harmful branched chain fatty acids (BCFA) fermentation products, but supported lactobacilli growth and increase in lactic acid production. Potato lintner starch had the greatest effect. Insoluble carbohydrates also suppressed production of SCFAs as compared to the control medium. Importantly, potato lintner starch most efficiently suppressed the ratio between the Bacteroidetes and Firmicutes and suppressed growth of bifidobacteria, Enterobactericeae and Faecalibacterium prausnizii. Degradative resistance of the lintner starch were linked to these effects. Problems using continuous fermentation systems for insoluble prebiotics and interpretation of batch fermentation data are discussed.

AB - Dietary fiber and slow carbohydrates can have differential beneficial effects on gut microbial composition and metabolism. Insoluble substrates such as RS cannot be used in continuous simulation systems and we tested an in vitro batch fermentation system for possible prebiotic effects using waxy maize starch granules, pectin-rich potato fiber, and potato lintner starch tested with human fresh fecal microbiota spiked with the probiotic Lactobacillus acidophilus NCFM. Microbial quantification by real-time polymerase chain-reaction (qRT-PCR) revealed that Bacteroidetes was specifically suppressed by each insoluble carbohydrate resulting in a clear decrease in the ratio of Bacteroidetes and Firmicutes. Notably, all carbohydrates tested appeared to block the formation of the potentially harmful branched chain fatty acids (BCFA) fermentation products, but supported lactobacilli growth and increase in lactic acid production. Potato lintner starch had the greatest effect. Insoluble carbohydrates also suppressed production of SCFAs as compared to the control medium. Importantly, potato lintner starch most efficiently suppressed the ratio between the Bacteroidetes and Firmicutes and suppressed growth of bifidobacteria, Enterobactericeae and Faecalibacterium prausnizii. Degradative resistance of the lintner starch were linked to these effects. Problems using continuous fermentation systems for insoluble prebiotics and interpretation of batch fermentation data are discussed.

U2 - 10.1002/star.201200091

DO - 10.1002/star.201200091

M3 - Journal article

SN - 0038-9056

VL - 65

SP - 346

EP - 353

JO - Starch

JF - Starch

IS - 3-4

ER -

Comparative fermentation of insoluble carbohydrates in an in vitro human feces model spiked with Lactobacillus acidophilus NCFM

Abstract

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