Cellular Delivery of Peptide Nucleic Acids (PNAs)

Takehiko Shiraishi, Peter E. Nielsen

17 Citations (Scopus)

Abstract

Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.

Original languageEnglish
Title of host publicationPeptide Nucleic Acids : Methods and Protocols
EditorsPeter E Nielsen, D.H. Appella
Number of pages16
Volume1050
Place of Publicationheidelberg
PublisherHumana Press
Publication date2014
Pages193-205
Chapter16
ISBN (Print)978-1-62703-552-1
Publication statusPublished - 2014
SeriesMethods in Molecular Biology
Volume1050
ISSN1064-3745

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