Cellular Delivery of Peptide Nucleic Acids (PNAs)

Takehiko Shiraishi, Peter E. Nielsen

17 Citationer (Scopus)

Abstract

Cellular delivery methods are a prerequisite for cellular studies with PNA. This chapter describes PNA cellular delivery using cell-penetrating peptide (CPP)-PNA conjugates and transfection of PNA-ligand conjugates mediated by cationic lipids. Furthermore, two endosomolytic procedures employing chloroquine treatment or photochemical internalization (PCI) for significantly improving PNA delivery efficacy are described.

OriginalsprogEngelsk
TitelPeptide Nucleic Acids : Methods and Protocols
RedaktørerPeter E Nielsen, D.H. Appella
Antal sider16
Vol/bind1050
Udgivelsesstedheidelberg
ForlagHumana Press
Publikationsdato2014
Sider193-205
Kapitel16
ISBN (Trykt)978-1-62703-552-1
StatusUdgivet - 2014
NavnMethods in Molecular Biology
Vol/bind1050
ISSN1064-3745

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