Abstract
In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
| Original language | English |
|---|---|
| Journal | Cell Cycle |
| Volume | 2 |
| Issue number | 5 |
| Pages (from-to) | 479-83 |
| Number of pages | 5 |
| ISSN | 1538-4101 |
| Publication status | Published - 10 Sept 2003 |
Keywords
- Cell Cycle
- DNA Damage
- DNA Repair
- DNA-Binding Proteins
- Rad52 DNA Repair and Recombination Protein
- Recombination, Genetic
- S Phase
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Journal Article
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, P.H.S.