Cell cycle-regulated centers of DNA double-strand break repair

Michael Lisby; Adriana Antúnez de Mayolo; Uffe H Mortensen; Rodney Rothstein

Cell cycle-regulated centers of DNA double-strand break repair

Michael Lisby, Adriana Antúnez de Mayolo, Uffe H Mortensen, Rodney Rothstein

Functional genomics

53 Citationer (Scopus)

Abstract

In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.

Originalsprog	Engelsk
Tidsskrift	Cell Cycle
Vol/bind	2
Udgave nummer	5
Sider (fra-til)	479-83
Antal sider	5
ISSN	1538-4101
Status	Udgivet - 10 sep. 2003

Citationsformater

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title = "Cell cycle-regulated centers of DNA double-strand break repair",

abstract = "In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.",

keywords = "Cell Cycle, DNA Damage, DNA Repair, DNA-Binding Proteins, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.",

author = "Michael Lisby and {Ant{\'u}nez de Mayolo}, Adriana and Mortensen, {Uffe H} and Rodney Rothstein",

year = "2003",

month = sep,

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language = "English",

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TY - JOUR

T1 - Cell cycle-regulated centers of DNA double-strand break repair

AU - Lisby, Michael

AU - Antúnez de Mayolo, Adriana

AU - Mortensen, Uffe H

AU - Rothstein, Rodney

PY - 2003/9/10

Y1 - 2003/9/10

N2 - In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.

AB - In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.

KW - Cell Cycle

KW - DNA Damage

KW - DNA Repair

KW - DNA-Binding Proteins

KW - Rad52 DNA Repair and Recombination Protein

KW - Recombination, Genetic

KW - S Phase

KW - Saccharomyces cerevisiae

KW - Saccharomyces cerevisiae Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, P.H.S.

M3 - Journal article

C2 - 12963848

SN - 1538-4101

VL - 2

SP - 479

EP - 483

JO - Cell Cycle

JF - Cell Cycle

IS - 5

ER -

Cell cycle-regulated centers of DNA double-strand break repair

Abstract

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