C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

Song Lin Chua; Krishnakumar Sivakumar; Morten Levin Rybtke; Mingjun Yuan; Jens Bo Andersen; Thomas E. Nielsen; Michael Givskov; Tim Tolker-Nielsen; Bin Cao; Staffan Kjelleberg; Liang Yang

doi:10.1038/srep10052

C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

Song Lin Chua, Krishnakumar Sivakumar, Morten Levin Rybtke, Mingjun Yuan, Jens Bo Andersen, Thomas E. Nielsen, Michael Givskov, Tim Tolker-Nielsen, Bin Cao, Staffan Kjelleberg, Liang Yang

36 Citations (Scopus)

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Abstract

Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO₃^2-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO₃^2- further increased P. aeruginosa biofilm formation and resistance to TeO₃^2-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO₃^2- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO₃^2-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

Original language	English
Article number	10052
Journal	Scientific Reports
Volume	5
Number of pages	13
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep10052
Publication status	Published - 20 May 2015

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C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growthFinal published version, 1.09 MB

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title = "C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth",

abstract = "Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa {"}sadC {"}siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.",

author = "Chua, {Song Lin} and Krishnakumar Sivakumar and Rybtke, {Morten Levin} and Mingjun Yuan and Andersen, {Jens Bo} and Nielsen, {Thomas E.} and Michael Givskov and Tim Tolker-Nielsen and Bin Cao and Staffan Kjelleberg and Liang Yang",

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doi = "10.1038/srep10052",

language = "English",

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T1 - C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

AU - Chua, Song Lin

AU - Sivakumar, Krishnakumar

AU - Rybtke, Morten Levin

AU - Yuan, Mingjun

AU - Andersen, Jens Bo

AU - Nielsen, Thomas E.

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Cao, Bin

AU - Kjelleberg, Staffan

AU - Yang, Liang

PY - 2015/5/20

Y1 - 2015/5/20

N2 - Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

AB - Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

U2 - 10.1038/srep10052

DO - 10.1038/srep10052

M3 - Journal article

C2 - 25992876

SN - 2045-2322

VL - 5

JO - Scientific Reports

JF - Scientific Reports

M1 - 10052

ER -

C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

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