C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

TY - JOUR

T1 - C-di-GMP regulates Pseudomonas aeruginosa stress response to tellurite during both planktonic and biofilm modes of growth

AU - Chua, Song Lin

AU - Sivakumar, Krishnakumar

AU - Rybtke, Morten Levin

AU - Yuan, Mingjun

AU - Andersen, Jens Bo

AU - Nielsen, Thomas E.

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Cao, Bin

AU - Kjelleberg, Staffan

AU - Yang, Liang

PY - 2015/5/20

Y1 - 2015/5/20

N2 - Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

AB - Stress response plays an important role on microbial adaptation under hostile environmental conditions. It is generally unclear how the signaling transduction pathway mediates a stress response in planktonic and biofilm modes of microbial communities simultaneously. Here, we showed that metalloid tellurite (TeO32-) exposure induced the intracellular content of the secondary messenger cyclic di-GMP (c-di-GMP) of Pseudomonas aeruginosa. Two diguanylate cyclases (DGCs), SadC and SiaD, were responsible for the increased intracellular content of c-di-GMP. Enhanced c-di-GMP levels by TeO32- further increased P. aeruginosa biofilm formation and resistance to TeO32-. P. aeruginosa "sadC "siaD and PAO1/p lac-yhjH mutants with low intracellular c-di-GMP content were more sensitive to TeO32- exposure and had low relative fitness compared to the wild-type PAO1 planktonic and biofilm cultures exposed to TeO32-. Our study provided evidence that c-di-GMP level can play an important role in mediating stress response in microbial communities during both planktonic and biofilm modes of growth.

U2 - 10.1038/srep10052

DO - 10.1038/srep10052

M3 - Journal article

C2 - 25992876

SN - 2045-2322

VL - 5

JO - Scientific Reports

JF - Scientific Reports

M1 - 10052

ER -