Bursting the bubble on bacterial biofilms: a flow cell methodology

Shanika A Crusz; Roman Popat; Morten Theil Rybtke; Miguel Cámara; Michael Givskov; Tim Tolker-Nielsen; Stephen P Diggle; Paul Williams

doi:10.1080/08927014.2012.716044

Bursting the bubble on bacterial biofilms: a flow cell methodology

Shanika A Crusz, Roman Popat, Morten Theil Rybtke, Miguel Cámara, Michael Givskov, Tim Tolker-Nielsen, Stephen P Diggle, Paul Williams

Department of Immunology and Microbiology

66 Citations (Scopus)

Abstract

The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.

Original language	English
Journal	Biofouling
Volume	28
Issue number	8
Pages (from-to)	835-842
Number of pages	8
ISSN	0892-7014
DOIs	https://doi.org/10.1080/08927014.2012.716044
Publication status	Published - Jul 2012

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abstract = "The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.",

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AU - Crusz, Shanika A

AU - Popat, Roman

AU - Rybtke, Morten Theil

AU - Cámara, Miguel

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Diggle, Stephen P

AU - Williams, Paul

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AB - The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.

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Bursting the bubble on bacterial biofilms: a flow cell methodology

Abstract

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