Bursting the bubble on bacterial biofilms: a flow cell methodology

Shanika A Crusz; Roman Popat; Morten Theil Rybtke; Miguel Cámara; Michael Givskov; Tim Tolker-Nielsen; Stephen P Diggle; Paul Williams

doi:10.1080/08927014.2012.716044

Bursting the bubble on bacterial biofilms: a flow cell methodology

Shanika A Crusz, Roman Popat, Morten Theil Rybtke, Miguel Cámara, Michael Givskov, Tim Tolker-Nielsen, Stephen P Diggle, Paul Williams

LUKKET: Institut for Immunologi og Mikrobiologi

66 Citationer (Scopus)

Abstract

The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.

Originalsprog	Engelsk
Tidsskrift	Biofouling
Vol/bind	28
Udgave nummer	8
Sider (fra-til)	835-842
Antal sider	8
ISSN	0892-7014
DOI	https://doi.org/10.1080/08927014.2012.716044
Status	Udgivet - jul. 2012

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10.1080/08927014.2012.716044

Citationsformater

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abstract = "The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.",

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T2 - a flow cell methodology

AU - Crusz, Shanika A

AU - Popat, Roman

AU - Rybtke, Morten Theil

AU - Cámara, Miguel

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

AU - Diggle, Stephen P

AU - Williams, Paul

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AB - The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems, accompanied by a detailed guide to the assembly of the apparatus including the description of new modifications which enhance its performance. As such, this is an essential guide for the novice biofilm researcher as well as providing valuable trouble-shooting techniques for even the most experienced laboratories. The adoption of a common and reliable methodology amongst researchers would enable findings to be shared and replicated amongst the biofilm research community, with the overall aim of advancing understanding and management of these complex and widespread bacterial communities.

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DO - 10.1080/08927014.2012.716044

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EP - 842

JO - Biofouling

JF - Biofouling

IS - 8

ER -

Bursting the bubble on bacterial biofilms: a flow cell methodology

Abstract

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