Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs

Yanqi Li; Duc Ninh Nguyen; Karina Obelitz-Ryom; Anders D. Andersen; Thomas Thymann; Dereck E.W. Chatterton; Stig Purup; Anne B. Heckmann; Stine B. Bering; Per T. Sangild

doi:10.1097/mpg.0000000000001699

Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs

Yanqi Li^*, Duc Ninh Nguyen, Karina Obelitz-Ryom, Anders D. Andersen, Thomas Thymann, Dereck E.W. Chatterton, Stig Purup, Anne B. Heckmann, Stine B. Bering, Per T. Sangild

^*Corresponding author for this work

14 Citations (Scopus)

Abstract

Objective: Formula feeding is associated with compromised intestinal health in preterm neonates compared with maternal milk, but the mechanisms behind this are unclear. We hypothesized that the use of maltodextrin and whey protein concentrates (WPCs) with reduced bioactivity owing to thermal processing are important factors. Method: Ninety-two cesarean-delivered preterm pigs were fed increasing doses of formulas for 5 days (24-120mL kg ^-1 day ^-1 ). In experiment 1, 4 groups of pigs (n=15-16) were fed lactose- or maltodextrin-dominant formulas (lactose/maltodextrin ratios 3:1 or 1:3, respectively), containing WPC with either high or low levels of IgG (WPC1 or WPC2, respectively). In experiment 2, 2 groups of pigs (n=15-16) were fed lactose-dominant formulas with either a bioactive WPC (BioWPC, produced by reduced thermal-processing) or a conventional WPC (ConWPC). Results: In experiment 1, pigs fed formula with WPC1 had higher villi, hexose absorption, and lactase activity in small intestine, relative to WPC2, but predominantly with the lactose-dominant formula (all P<0.05). In experiment 2, the BioWPC product had higher bioactivity, as indicated by higher IgG, lactoferrin, and TGF-b2 levels, and better enterocyte proliferation in vitro. Pigs fed the BioWPC formula showed better feeding tolerance and higher intestinal villi and lactase activity (all P<0.05). The BioWPC formula-fed pigs also had greater physical activity (P<0.05 on day 4) and tended to show improved hexose absorption and decreased gut permeability (both P≤0.09). Conclusions: Infant formulas containing lactose as the main carbohydrate, and WPC with reduced thermal processing, may support gut maturation and health in sensitive, preterm neonates.

Original language	English
Journal	Journal of Pediatric Gastroenterology and Nutrition
Volume	66
Issue number	1
Pages (from-to)	128-134
Number of pages	7
ISSN	0277-2116
DOIs	https://doi.org/10.1097/mpg.0000000000001699
Publication status	Published - 1 Jan 2018

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Li, Y., Nguyen, D. N., Obelitz-Ryom, K., Andersen, A. D., Thymann, T., Chatterton, D. E. W., Purup, S., Heckmann, A. B., Bering, S. B., & Sangild, P. T. (2018). Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs. Journal of Pediatric Gastroenterology and Nutrition, 66(1), 128-134. https://doi.org/10.1097/mpg.0000000000001699

@article{3c1cf9e7cbfd45fb8612eb3f0ee3fa37,

title = "Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs",

abstract = " Objective: Formula feeding is associated with compromised intestinal health in preterm neonates compared with maternal milk, but the mechanisms behind this are unclear. We hypothesized that the use of maltodextrin and whey protein concentrates (WPCs) with reduced bioactivity owing to thermal processing are important factors. Method: Ninety-two cesarean-delivered preterm pigs were fed increasing doses of formulas for 5 days (24-120mL kg -1 day -1 ). In experiment 1, 4 groups of pigs (n=15-16) were fed lactose- or maltodextrin-dominant formulas (lactose/maltodextrin ratios 3:1 or 1:3, respectively), containing WPC with either high or low levels of IgG (WPC1 or WPC2, respectively). In experiment 2, 2 groups of pigs (n=15-16) were fed lactose-dominant formulas with either a bioactive WPC (BioWPC, produced by reduced thermal-processing) or a conventional WPC (ConWPC). Results: In experiment 1, pigs fed formula with WPC1 had higher villi, hexose absorption, and lactase activity in small intestine, relative to WPC2, but predominantly with the lactose-dominant formula (all P<0.05). In experiment 2, the BioWPC product had higher bioactivity, as indicated by higher IgG, lactoferrin, and TGF-b2 levels, and better enterocyte proliferation in vitro. Pigs fed the BioWPC formula showed better feeding tolerance and higher intestinal villi and lactase activity (all P<0.05). The BioWPC formula-fed pigs also had greater physical activity (P<0.05 on day 4) and tended to show improved hexose absorption and decreased gut permeability (both P≤0.09). Conclusions: Infant formulas containing lactose as the main carbohydrate, and WPC with reduced thermal processing, may support gut maturation and health in sensitive, preterm neonates. ",

author = "Yanqi Li and Nguyen, {Duc Ninh} and Karina Obelitz-Ryom and Andersen, {Anders D.} and Thomas Thymann and Chatterton, {Dereck E.W.} and Stig Purup and Heckmann, {Anne B.} and Bering, {Stine B.} and Sangild, {Per T.}",

year = "2018",

month = jan,

day = "1",

doi = "10.1097/mpg.0000000000001699",

language = "English",

volume = "66",

pages = "128--134",

journal = "Journal of Pediatric Gastroenterology and Nutrition",

issn = "0277-2116",

publisher = "Lippincott Williams & Wilkins",

number = "1",

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TY - JOUR

T1 - Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs

AU - Li, Yanqi

AU - Nguyen, Duc Ninh

AU - Obelitz-Ryom, Karina

AU - Andersen, Anders D.

AU - Thymann, Thomas

AU - Chatterton, Dereck E.W.

AU - Purup, Stig

AU - Heckmann, Anne B.

AU - Bering, Stine B.

AU - Sangild, Per T.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objective: Formula feeding is associated with compromised intestinal health in preterm neonates compared with maternal milk, but the mechanisms behind this are unclear. We hypothesized that the use of maltodextrin and whey protein concentrates (WPCs) with reduced bioactivity owing to thermal processing are important factors. Method: Ninety-two cesarean-delivered preterm pigs were fed increasing doses of formulas for 5 days (24-120mL kg -1 day -1 ). In experiment 1, 4 groups of pigs (n=15-16) were fed lactose- or maltodextrin-dominant formulas (lactose/maltodextrin ratios 3:1 or 1:3, respectively), containing WPC with either high or low levels of IgG (WPC1 or WPC2, respectively). In experiment 2, 2 groups of pigs (n=15-16) were fed lactose-dominant formulas with either a bioactive WPC (BioWPC, produced by reduced thermal-processing) or a conventional WPC (ConWPC). Results: In experiment 1, pigs fed formula with WPC1 had higher villi, hexose absorption, and lactase activity in small intestine, relative to WPC2, but predominantly with the lactose-dominant formula (all P<0.05). In experiment 2, the BioWPC product had higher bioactivity, as indicated by higher IgG, lactoferrin, and TGF-b2 levels, and better enterocyte proliferation in vitro. Pigs fed the BioWPC formula showed better feeding tolerance and higher intestinal villi and lactase activity (all P<0.05). The BioWPC formula-fed pigs also had greater physical activity (P<0.05 on day 4) and tended to show improved hexose absorption and decreased gut permeability (both P≤0.09). Conclusions: Infant formulas containing lactose as the main carbohydrate, and WPC with reduced thermal processing, may support gut maturation and health in sensitive, preterm neonates.

AB - Objective: Formula feeding is associated with compromised intestinal health in preterm neonates compared with maternal milk, but the mechanisms behind this are unclear. We hypothesized that the use of maltodextrin and whey protein concentrates (WPCs) with reduced bioactivity owing to thermal processing are important factors. Method: Ninety-two cesarean-delivered preterm pigs were fed increasing doses of formulas for 5 days (24-120mL kg -1 day -1 ). In experiment 1, 4 groups of pigs (n=15-16) were fed lactose- or maltodextrin-dominant formulas (lactose/maltodextrin ratios 3:1 or 1:3, respectively), containing WPC with either high or low levels of IgG (WPC1 or WPC2, respectively). In experiment 2, 2 groups of pigs (n=15-16) were fed lactose-dominant formulas with either a bioactive WPC (BioWPC, produced by reduced thermal-processing) or a conventional WPC (ConWPC). Results: In experiment 1, pigs fed formula with WPC1 had higher villi, hexose absorption, and lactase activity in small intestine, relative to WPC2, but predominantly with the lactose-dominant formula (all P<0.05). In experiment 2, the BioWPC product had higher bioactivity, as indicated by higher IgG, lactoferrin, and TGF-b2 levels, and better enterocyte proliferation in vitro. Pigs fed the BioWPC formula showed better feeding tolerance and higher intestinal villi and lactase activity (all P<0.05). The BioWPC formula-fed pigs also had greater physical activity (P<0.05 on day 4) and tended to show improved hexose absorption and decreased gut permeability (both P≤0.09). Conclusions: Infant formulas containing lactose as the main carbohydrate, and WPC with reduced thermal processing, may support gut maturation and health in sensitive, preterm neonates.

U2 - 10.1097/mpg.0000000000001699

DO - 10.1097/mpg.0000000000001699

M3 - Journal article

C2 - 28753186

AN - SCOPUS:85026473237

SN - 0277-2116

VL - 66

SP - 128

EP - 134

JO - Journal of Pediatric Gastroenterology and Nutrition

JF - Journal of Pediatric Gastroenterology and Nutrition

IS - 1

ER -

Bioactive Whey Protein Concentrate and Lactose Stimulate Gut Function in Formula-Fed Preterm Pigs

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