Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Michael Stangegaard; Mads Jørgensen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2009.08.001

Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Michael Stangegaard, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

Section of Forensic Genetics

5 Citations (Scopus)

Abstract

We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025

Original language	English
Title of host publication	Progress in Forensic Genetics 13 : Proceedings of the 23rd International ISFG Congress
Editors	Niels Morling
Number of pages	2
Publisher	Elsevier
Publication date	2009
Pages	69-70
DOIs	https://doi.org/10.1016/j.fsigss.2009.08.001
Publication status	Published - 2009
Event	23rd International ISFG Congress - Buenos Aires, Argentina Duration: 14 Sept 2009 → 18 Sept 2009

Conference

Conference	23rd International ISFG Congress
Country/Territory	Argentina
City	Buenos Aires
Period	14/09/2009 → 18/09/2009

Series	Forensic Science International: Genetics Supplement Series
Number	1
Volume	2
ISSN	1875-1768

Access to Document

10.1016/j.fsigss.2009.08.001

Cite this

Stangegaard, M., Jørgensen, M., Hansen, A. J., & Morling, N. (2009). Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. In N. Morling (Ed.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress (pp. 69-70). Elsevier. https://doi.org/10.1016/j.fsigss.2009.08.001

Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. / Stangegaard, Michael; Jørgensen, Mads; Hansen, Anders Johannes et al.
Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. ed. / Niels Morling. Elsevier, 2009. p. 69-70 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Stangegaard, M, Jørgensen, M, Hansen, AJ & Morling, N 2009, Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. in N Morling (ed.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier, Forensic Science International: Genetics Supplement Series, no. 1, vol. 2, pp. 69-70, 23rd International ISFG Congress, Buenos Aires, Argentina, 14/09/2009. https://doi.org/10.1016/j.fsigss.2009.08.001

Stangegaard M, Jørgensen M, Hansen AJ , Morling N. Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. In Morling N, editor, Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier. 2009. p. 69-70. (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2). doi: 10.1016/j.fsigss.2009.08.001

Stangegaard, Michael ; Jørgensen, Mads ; Hansen, Anders Johannes et al. / Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. editor / Niels Morling. Elsevier, 2009. pp. 69-70 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

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