Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Michael Stangegaard; Mads Jørgensen; Anders Johannes Hansen; Niels Morling

doi:doi:10.1016/j.fsigss.2009.08.001

Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation

Michael Stangegaard, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

Afdeling for Retsgenetik

5 Citationer (Scopus)

Abstract

We have validated and implemented a protocol for DNA extraction from various types of biological materials using a Qiagen BioRobot EZ1 Workstation. The sample materials included whole blood, blood from deceased, buccal cells on Omni swabs and FTA Cards, blood on FTA Cards and cotton swabs, and muscle biopsies. The DNA extraction was validated according to EN/ISO 17025 for the STR kits AmpFlSTR« Identifiler« and AmpFlSTR« Yfiler« (Applied Biosystems). Of 298 samples extracted, 11 (4%) did not yield acceptable results. In conclusion, we have demonstrated that extraction of DNA from various types of biological material can be performed quickly and without the use of hazardous chemicals, and that the DNA may be successfully STR typed according to the requirements of forensic genetic investigations accredited according to EN/ISO 17025

Originalsprog	Engelsk
Titel	Progress in Forensic Genetics 13 : Proceedings of the 23rd International ISFG Congress
Redaktører	Niels Morling
Antal sider	2
Forlag	Elsevier
Publikationsdato	2009
Sider	69-70
DOI	https://doi.org/doi:10.1016/j.fsigss.2009.08.001
Status	Udgivet - 2009
Begivenhed	23rd International ISFG Congress - Buenos Aires, Argentina Varighed: 14 sep. 2009 → 18 sep. 2009

Konference

Konference	23rd International ISFG Congress
Land/Område	Argentina
By	Buenos Aires
Periode	14/09/2009 → 18/09/2009

Navn	Forensic Science International: Genetics Supplement Series
Nummer	1
Vol/bind	2
ISSN	1875-1768

Adgang til dokumentet

doi:10.1016/j.fsigss.2009.08.001

Citationsformater

Stangegaard, M., Jørgensen, M., Hansen, A. J., & Morling, N. (2009). Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. I N. Morling (red.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress (s. 69-70). Elsevier. Forensic Science International: Genetics Supplement Series Bind 2 Nr. 1 https://doi.org/doi:10.1016/j.fsigss.2009.08.001

Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. / Stangegaard, Michael; Jørgensen, Mads; Hansen, Anders Johannes et al.

Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. red. / Niels Morling. Elsevier, 2009. s. 69-70 (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2).

Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › peer review

Stangegaard, M, Jørgensen, M, Hansen, AJ & Morling, N 2009, Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. i N Morling (red.), Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier, Forensic Science International: Genetics Supplement Series, nr. 1, bind 2, s. 69-70, 23rd International ISFG Congress, Buenos Aires, Argentina, 14/09/2009. https://doi.org/doi:10.1016/j.fsigss.2009.08.001

Stangegaard M, Jørgensen M, Hansen AJ , Morling N. Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. I Morling N, red., Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. Elsevier. 2009. s. 69-70. (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2). doi: doi:10.1016/j.fsigss.2009.08.001

Stangegaard, Michael ; Jørgensen, Mads ; Hansen, Anders Johannes et al. / Automated extraction of DNA from reference samples from various types of biological materials on the Qiagen BioRobot EZ1 Workstation. Progress in Forensic Genetics 13: Proceedings of the 23rd International ISFG Congress. red. / Niels Morling. Elsevier, 2009. s. 69-70 (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2).

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