Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler

Michael Stangegaard; Tobias G. Frøslev; Rune Frank-Hansen; Susan Laursen; Mads Jørgensen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2009.07.002

Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler

Michael Stangegaard, Tobias G. Frøslev, Rune Frank-Hansen, Susan Laursen, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

Section of Forensic Genetics

7 Citations (Scopus)

Abstract

We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025

Original language	English
Title of host publication	Progress in Forensic Genetics 13 : Proceedings from the 23rd International ISFG Congress
Editors	Niels Morling
Number of pages	3
Volume	E-Pub
Publisher	Elsevier
Publication date	2009
Pages	74-76
DOIs	https://doi.org/10.1016/j.fsigss.2009.07.002
Publication status	Published - 2009
Event	23rd International ISFG Congress - Buenos Aires, Argentina Duration: 14 Sept 2009 → 18 Sept 2009

Conference

Conference	23rd International ISFG Congress
Country/Territory	Argentina
City	Buenos Aires
Period	14/09/2009 → 18/09/2009

Series	Forensic Science International: Genetics Supplement Series
Number	1
Volume	2
ISSN	1875-1768

Access to Document

10.1016/j.fsigss.2009.07.002

Cite this

Stangegaard, M., Frøslev, T. G., Frank-Hansen, R., Laursen, S., Jørgensen, M., Hansen, A. J., & Morling, N. (2009). Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. In N. Morling (Ed.), Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress (Vol. E-Pub, pp. 74-76). Elsevier. https://doi.org/10.1016/j.fsigss.2009.07.002

Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. / Stangegaard, Michael; Frøslev, Tobias G.; Frank-Hansen, Rune et al.
Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. ed. / Niels Morling. Vol. E-Pub Elsevier, 2009. p. 74-76 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Stangegaard, M, Frøslev, TG, Frank-Hansen, R, Laursen, S, Jørgensen, M, Hansen, AJ & Morling, N 2009, Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. in N Morling (ed.), Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. vol. E-Pub, Elsevier, Forensic Science International: Genetics Supplement Series, no. 1, vol. 2, pp. 74-76, 23rd International ISFG Congress, Buenos Aires, Argentina, 14/09/2009. https://doi.org/10.1016/j.fsigss.2009.07.002

Stangegaard M, Frøslev TG, Frank-Hansen R, Laursen S, Jørgensen M, Hansen AJ et al. Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. In Morling N, editor, Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. Vol. E-Pub. Elsevier. 2009. p. 74-76. (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2). doi: 10.1016/j.fsigss.2009.07.002

Stangegaard, Michael ; Frøslev, Tobias G. ; Frank-Hansen, Rune et al. / Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. editor / Niels Morling. Vol. E-Pub Elsevier, 2009. pp. 74-76 (Forensic Science International: Genetics Supplement Series; No. 1, Vol. 2).

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abstract = "We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025",

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