Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler

Michael Stangegaard; Tobias G. Frøslev; Rune Frank-Hansen; Susan Laursen; Mads Jørgensen; Anders Johannes Hansen; Niels Morling

doi:doi:10.1016/j.fsigss.2009.07.002

Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler

Michael Stangegaard, Tobias G. Frøslev, Rune Frank-Hansen, Susan Laursen, Mads Jørgensen, Anders Johannes Hansen, Niels Morling

Afdeling for Retsgenetik

7 Citationer (Scopus)

Abstract

We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025

Originalsprog	Engelsk
Titel	Progress in Forensic Genetics 13 : Proceedings from the 23rd International ISFG Congress
Redaktører	Niels Morling
Antal sider	3
Vol/bind	E-Pub
Forlag	Elsevier
Publikationsdato	2009
Sider	74-76
DOI	https://doi.org/doi:10.1016/j.fsigss.2009.07.002
Status	Udgivet - 2009
Begivenhed	23rd International ISFG Congress - Buenos Aires, Argentina Varighed: 14 sep. 2009 → 18 sep. 2009

Konference

Konference	23rd International ISFG Congress
Land/Område	Argentina
By	Buenos Aires
Periode	14/09/2009 → 18/09/2009

Navn	Forensic Science International: Genetics Supplement Series
Nummer	1
Vol/bind	2
ISSN	1875-1768

Adgang til dokumentet

doi:10.1016/j.fsigss.2009.07.002

Citationsformater

Stangegaard, M., Frøslev, T. G., Frank-Hansen, R., Laursen, S., Jørgensen, M., Hansen, A. J., & Morling, N. (2009). Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. I N. Morling (red.), Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress (Bind E-Pub, s. 74-76). Elsevier. Forensic Science International: Genetics Supplement Series Bind 2 Nr. 1 https://doi.org/doi:10.1016/j.fsigss.2009.07.002

Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. / Stangegaard, Michael; Frøslev, Tobias G.; Frank-Hansen, Rune et al.

Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. red. / Niels Morling. Bind E-Pub Elsevier, 2009. s. 74-76 (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2).

Publikation: Bidrag til bog/antologi/rapport › Konferencebidrag i proceedings › Forskning › peer review

Stangegaard, M, Frøslev, TG, Frank-Hansen, R, Laursen, S, Jørgensen, M, Hansen, AJ & Morling, N 2009, Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. i N Morling (red.), Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. bind E-Pub, Elsevier, Forensic Science International: Genetics Supplement Series, nr. 1, bind 2, s. 74-76, 23rd International ISFG Congress, Buenos Aires, Argentina, 14/09/2009. https://doi.org/doi:10.1016/j.fsigss.2009.07.002

Stangegaard M, Frøslev TG, Frank-Hansen R, Laursen S, Jørgensen M, Hansen AJ et al. Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. I Morling N, red., Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. Bind E-Pub. Elsevier. 2009. s. 74-76. (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2). doi: doi:10.1016/j.fsigss.2009.07.002

Stangegaard, Michael ; Frøslev, Tobias G. ; Frank-Hansen, Rune et al. / Automated extraction of DNA and PCR setup using a Tecan Freedom EVO^® liquid handler. Progress in Forensic Genetics 13: Proceedings from the 23rd International ISFG Congress. red. / Niels Morling. Bind E-Pub Elsevier, 2009. s. 74-76 (Forensic Science International: Genetics Supplement Series; Nr. 1, Bind 2).

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abstract = "We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025",

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AU - Stangegaard, Michael

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AU - Laursen, Susan

AU - Jørgensen, Mads

AU - Hansen, Anders Johannes

AU - Morling, Niels

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AB - We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO« liquid handler mounted with a Te-MagS(TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract« DNA Mini M48 kit. The DNA was amplified using AmpFlSTR« Identifiler«, Y-filer« (Applied Biosystems), GenePrint« FFFL and PowerPlex« Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025

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