Automated addition of Chelex solution to tubes containing trace items

Michael Stangegaard; Thomas Møller Hansen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2011.08.082

Automated addition of Chelex solution to tubes containing trace items

Michael Stangegaard, Thomas Møller Hansen, Anders Johannes Hansen, Niels Morling

1 Citation (Scopus)

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Abstract

Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

Original language	English
Journal	Forensic Science International: Genetics. Supplement Series
Volume	3
Issue number	1
Pages (from-to)	Pages e163-e164
Number of pages	2
ISSN	1875-1768
DOIs	https://doi.org/10.1016/j.fsigss.2011.08.082
Publication status	Published - Dec 2011

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10.1016/j.fsigss.2011.08.082

Automated addition of Chelex solution to tubes containing trace itemsFinal published version, 313 KB

http://www.fsigeneticssup.com/article/S1875176811000837/pdf

Cite this

@inproceedings{35ec96f3b9ca4e44a4cab3b3df4db42b,

title = "Automated addition of Chelex solution to tubes containing trace items",

abstract = "Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues",

author = "Michael Stangegaard and Hansen, {Thomas M{\o}ller} and Hansen, {Anders Johannes} and Niels Morling",

year = "2011",

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AU - Stangegaard, Michael

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AU - Hansen, Anders Johannes

AU - Morling, Niels

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N2 - Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

AB - Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

U2 - 10.1016/j.fsigss.2011.08.082

DO - 10.1016/j.fsigss.2011.08.082

M3 - Conference article

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SP - Pages e163-e164

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

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