Automated addition of Chelex solution to tubes containing trace items

Michael Stangegaard; Thomas Møller Hansen; Anders Johannes Hansen; Niels Morling

doi:10.1016/j.fsigss.2011.08.082

Automated addition of Chelex solution to tubes containing trace items

Michael Stangegaard, Thomas Møller Hansen, Anders Johannes Hansen, Niels Morling

1 Citationer (Scopus)

1985 Downloads (Pure)

Abstract

Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

Originalsprog	Engelsk
Tidsskrift	Forensic Science International: Genetics. Supplement Series
Vol/bind	3
Udgave nummer	1
Sider (fra-til)	Pages e163-e164
Antal sider	2
ISSN	1875-1768
DOI	https://doi.org/10.1016/j.fsigss.2011.08.082
Status	Udgivet - dec. 2011

Adgang til dokumentet

10.1016/j.fsigss.2011.08.082

Automated addition of Chelex solution to tubes containing trace itemsForlagets udgivne version, 313 KB

http://www.fsigeneticssup.com/article/S1875176811000837/pdf

Citationsformater

@inproceedings{35ec96f3b9ca4e44a4cab3b3df4db42b,

title = "Automated addition of Chelex solution to tubes containing trace items",

abstract = "Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues",

author = "Michael Stangegaard and Hansen, {Thomas M{\o}ller} and Hansen, {Anders Johannes} and Niels Morling",

year = "2011",

month = dec,

doi = "10.1016/j.fsigss.2011.08.082",

language = "English",

volume = "3",

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journal = "Forensic Science International: Genetics. Supplement Series",

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AU - Stangegaard, Michael

AU - Hansen, Thomas Møller

AU - Hansen, Anders Johannes

AU - Morling, Niels

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N2 - Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

AB - Extraction of DNA from trace items for forensic genetic DNA typing using a manual Chelex based extraction protocol requires addition of Chelex solution to sample tubes containing trace items. Automated of addition of Chelex solution may be hampered by high viscosity of the solution and fast sedimentation rate of the Chelex beads. Here, we present a simple method that can be used on an Eppendorf epMotion liquid handler resolving these issues

U2 - 10.1016/j.fsigss.2011.08.082

DO - 10.1016/j.fsigss.2011.08.082

M3 - Conference article

SN - 1875-1768

VL - 3

SP - Pages e163-e164

JO - Forensic Science International: Genetics. Supplement Series

JF - Forensic Science International: Genetics. Supplement Series

IS - 1

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