Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Stephan Alexander Pless; Robin Y Kim; Christopher A Ahern; Harley T Kurata

doi:10.1113/jphysiol.2014.287714

Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Stephan Alexander Pless, Robin Y Kim, Christopher A Ahern, Harley T Kurata

4 Citations (Scopus)

Abstract

Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

Original language	English
Journal	The Journal of Physiology
Volume	593
Issue number	12
Pages (from-to)	2627–2634
Number of pages	8
ISSN	0022-3751
DOIs	https://doi.org/10.1113/jphysiol.2014.287714
Publication status	Published - 15 Jun 2015

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title = "Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology",

abstract = "Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.",

author = "Pless, {Stephan Alexander} and Kim, {Robin Y} and Ahern, {Christopher A} and Kurata, {Harley T}",

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AU - Kim, Robin Y

AU - Ahern, Christopher A

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AB - Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

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DO - 10.1113/jphysiol.2014.287714

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Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Abstract

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