Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Stephan Alexander Pless; Robin Y Kim; Christopher A Ahern; Harley T Kurata

doi:10.1113/jphysiol.2014.287714

Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Stephan Alexander Pless, Robin Y Kim, Christopher A Ahern, Harley T Kurata

4 Citationer (Scopus)

Abstract

Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

Originalsprog	Engelsk
Tidsskrift	The Journal of Physiology
Vol/bind	593
Udgave nummer	12
Sider (fra-til)	2627–2634
Antal sider	8
ISSN	0022-3751
DOI	https://doi.org/10.1113/jphysiol.2014.287714
Status	Udgivet - 15 jun. 2015

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10.1113/jphysiol.2014.287714

jphysiol.2014.287714Forlagets udgivne version, 451 KB

Citationsformater

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abstract = "Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.",

author = "Pless, {Stephan Alexander} and Kim, {Robin Y} and Ahern, {Christopher A} and Kurata, {Harley T}",

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AB - Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.

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DO - 10.1113/jphysiol.2014.287714

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Atom-by-atom engineering of voltage-gated ion channels: Magnified insights into function and pharmacology

Abstract

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