Advances in nucleic acid-based diagnostics of bacterial infections.

Kim B Barken; Janus A J Haagensen; Tim Tolker-Nielsen

doi:10.1016/j.cca.2007.07.004

Advances in nucleic acid-based diagnostics of bacterial infections.

Kim B Barken, Janus A J Haagensen, Tim Tolker-Nielsen

97 Citations (Scopus)

Abstract

Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.

Original language	English
Journal	Clinica Chimica Acta
Volume	384
Issue number	1-2
Pages (from-to)	1-11
Number of pages	10
ISSN	0009-8981
DOIs	https://doi.org/10.1016/j.cca.2007.07.004
Publication status	Published - 2007
Externally published	Yes

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10.1016/j.cca.2007.07.004

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title = "Advances in nucleic acid-based diagnostics of bacterial infections.",

abstract = "Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.",

author = "Barken, {Kim B} and Haagensen, {Janus A J} and Tim Tolker-Nielsen",

note = "Keywords: Bacterial Infections; DNA, Bacterial; Humans; In Situ Hybridization; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; RNA, Bacterial; RNA, Ribosomal, 16S",

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AU - Barken, Kim B

AU - Haagensen, Janus A J

AU - Tolker-Nielsen, Tim

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PY - 2007

Y1 - 2007

N2 - Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.

AB - Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.

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DO - 10.1016/j.cca.2007.07.004

M3 - Journal article

C2 - 17689512

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VL - 384

SP - 1

EP - 11

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

IS - 1-2

ER -

Advances in nucleic acid-based diagnostics of bacterial infections.

Abstract

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