TY - JOUR
T1 - Advances in nucleic acid-based diagnostics of bacterial infections.
AU - Barken, Kim B
AU - Haagensen, Janus A J
AU - Tolker-Nielsen, Tim
N1 - Keywords: Bacterial Infections; DNA, Bacterial; Humans; In Situ Hybridization; Oligonucleotide Array Sequence Analysis; Polymerase Chain Reaction; RNA, Bacterial; RNA, Ribosomal, 16S
PY - 2007
Y1 - 2007
N2 - Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.
AB - Methods for rapid detection of infectious bacteria and antimicrobial-resistant pathogens have evolved significantly over the last decade. Many of the new procedures are nucleic acid-based and replace conventional diagnostic methods like culturing which is time consuming especially with fastidious and slow growing microorganisms. The widespread use of antibiotics has resulted in an increased number of cases with resistant microorganisms such as methicillin-resistant Staphylococcus aureus, vancomycin resistant enterococci, and multidrug-resistant Mycobacterium tuberculosis. Rapid detection of these pathogens is important to isolate patients and prevent further spreading of the diseases. Newly developed diagnostic procedures are superior with respect to turnaround time, sensitivity and specificity. Methods like multiplex real time PCR and different array-based technologies offer the possibility of multiparameter assays where several pathogens and antibiotic resistance genes can be detected simultaneously.
U2 - 10.1016/j.cca.2007.07.004
DO - 10.1016/j.cca.2007.07.004
M3 - Journal article
C2 - 17689512
SN - 0009-8981
VL - 384
SP - 1
EP - 11
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -
