Activated human CD4 T cells express transporters for both cysteine and cystine

Trine Bøegh Levring; Ann Kathrine Hansen; Bodil Lisbeth Nielsen; Martin Kongsbak; Marina Rode von Essen; Anders Woetmann; Niels Odum; Charlotte Menné Bonefeld; Carsten Geisler

doi:10.1038/srep00266

Activated human CD4 T cells express transporters for both cysteine and cystine

Trine Bøegh Levring, Ann Kathrine Hansen, Bodil Lisbeth Nielsen, Martin Kongsbak, Marina Rode von Essen, Anders Woetmann, Niels Odum, Charlotte Menné Bonefeld, Carsten Geisler

Department of Immunology and Microbiology

42 Citations (Scopus)

Abstract

Because naïve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystine/cysteine is required and how T cells meet this requirement. We found that early activation of T cells is independent of exogenous cystine/cysteine, whereas T cell proliferation is strictly dependent of uptake of exogenous cystine/cysteine. Naïve T cells express no or very low levels of both cystine and cysteine transporters. However, we found that these transporters become strongly up-regulated during T cell activation and provide activated T cells with the required amount of cystine/cysteine needed for T cell proliferation. Thus, T cells are equipped with mechanisms that allow T cell activation and proliferation independently of cysteine generated by antigen presenting cells.

Original language	English
Article number	266
Journal	Scientific Reports
Volume	2
ISSN	2045-2322
DOIs	https://doi.org/10.1038/srep00266
Publication status	Published - 14 Feb 2012

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title = "Activated human CD4 T cells express transporters for both cysteine and cystine",

abstract = "Because na{\"i}ve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystine/cysteine is required and how T cells meet this requirement. We found that early activation of T cells is independent of exogenous cystine/cysteine, whereas T cell proliferation is strictly dependent of uptake of exogenous cystine/cysteine. Na{\"i}ve T cells express no or very low levels of both cystine and cysteine transporters. However, we found that these transporters become strongly up-regulated during T cell activation and provide activated T cells with the required amount of cystine/cysteine needed for T cell proliferation. Thus, T cells are equipped with mechanisms that allow T cell activation and proliferation independently of cysteine generated by antigen presenting cells.",

author = "Levring, {Trine B{\o}egh} and Hansen, {Ann Kathrine} and Nielsen, {Bodil Lisbeth} and Martin Kongsbak and {von Essen}, {Marina Rode} and Anders Woetmann and Niels Odum and Bonefeld, {Charlotte Menn{\'e}} and Carsten Geisler",

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T1 - Activated human CD4 T cells express transporters for both cysteine and cystine

AU - Levring, Trine Bøegh

AU - Hansen, Ann Kathrine

AU - Nielsen, Bodil Lisbeth

AU - Kongsbak, Martin

AU - von Essen, Marina Rode

AU - Woetmann, Anders

AU - Odum, Niels

AU - Bonefeld, Charlotte Menné

AU - Geisler, Carsten

PY - 2012/2/14

Y1 - 2012/2/14

N2 - Because naïve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystine/cysteine is required and how T cells meet this requirement. We found that early activation of T cells is independent of exogenous cystine/cysteine, whereas T cell proliferation is strictly dependent of uptake of exogenous cystine/cysteine. Naïve T cells express no or very low levels of both cystine and cysteine transporters. However, we found that these transporters become strongly up-regulated during T cell activation and provide activated T cells with the required amount of cystine/cysteine needed for T cell proliferation. Thus, T cells are equipped with mechanisms that allow T cell activation and proliferation independently of cysteine generated by antigen presenting cells.

AB - Because naïve T cells are unable to import cystine due to the absence of cystine transporters, it has been suggested that T cell activation is dependent on cysteine generated by antigen presenting cells. The aim of this study was to determine at which phases during T cell activation exogenous cystine/cysteine is required and how T cells meet this requirement. We found that early activation of T cells is independent of exogenous cystine/cysteine, whereas T cell proliferation is strictly dependent of uptake of exogenous cystine/cysteine. Naïve T cells express no or very low levels of both cystine and cysteine transporters. However, we found that these transporters become strongly up-regulated during T cell activation and provide activated T cells with the required amount of cystine/cysteine needed for T cell proliferation. Thus, T cells are equipped with mechanisms that allow T cell activation and proliferation independently of cysteine generated by antigen presenting cells.

U2 - 10.1038/srep00266

DO - 10.1038/srep00266

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SN - 2045-2322

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JO - Scientific Reports

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ER -

Activated human CD4 T cells express transporters for both cysteine and cystine

Abstract

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