A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform

C. Harrison; E. Musgrave-Brown; K. Bender; A. Carracedo; N. Morling; Peter M. Schneider; Denise Syndercombe-Court

doi:doi:10.1016/j.ics.2005.10.021

A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform

C. Harrison, E. Musgrave-Brown, K. Bender, A. Carracedo, N. Morling, Peter M. Schneider, Denise Syndercombe-Court

Section of Forensic Genetics

7 Citations (Scopus)

Abstract

Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform

Original language	English
Title of host publication	Progress in Forensic Genetics 11 : Proceedings of the 21st International ISFG Congress
Volume	1288
Publisher	Elsevier
Publication date	2006
Pages	52-54
DOIs	https://doi.org/doi:10.1016/j.ics.2005.10.021
Publication status	Published - 2006
Event	21st International ISFG Congress - Ponta Delgada, The Azores, Portugal Duration: 13 Sept 2005 → 16 Sept 2005

Conference

Conference	21st International ISFG Congress
Country/Territory	Portugal
City	Ponta Delgada, The Azores
Period	13/09/2005 → 16/09/2005

Series	ICS - International Congress Series

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doi:10.1016/j.ics.2005.10.021

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Harrison, C., Musgrave-Brown, E., Bender, K., Carracedo, A., Morling, N., Schneider, P. M., & Syndercombe-Court, D. (2006). A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform. In Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress (Vol. 1288, pp. 52-54). Elsevier. ICS - International Congress Series https://doi.org/doi:10.1016/j.ics.2005.10.021

A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform. / Harrison, C.; Musgrave-Brown, E.; Bender, K. et al.

Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress. Vol. 1288 Elsevier, 2006. p. 52-54 (ICS - International Congress Series).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harrison, C, Musgrave-Brown, E, Bender, K, Carracedo, A, Morling, N, Schneider, PM & Syndercombe-Court, D 2006, A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform. in Progress in Forensic Genetics 11: Proceedings of the 21st International ISFG Congress. vol. 1288, Elsevier, ICS - International Congress Series, pp. 52-54, 21st International ISFG Congress, Ponta Delgada, The Azores, Portugal, 13/09/2005. https://doi.org/doi:10.1016/j.ics.2005.10.021

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abstract = "Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform",

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AU - Schneider, Peter M.

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AB - Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform

U2 - doi:10.1016/j.ics.2005.10.021

DO - doi:10.1016/j.ics.2005.10.021

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PB - Elsevier

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Y2 - 13 September 2005 through 16 September 2005

ER -

A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform

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