TY - GEN
T1 - A sensitive issue: Pyrosequencing as a valuable forensic SNP typing platform
AU - Harrison, C.
AU - Musgrave-Brown, E.
AU - Bender, K.
AU - Carracedo, A.
AU - Morling, N.
AU - Schneider, Peter M.
AU - Syndercombe-Court, Denise
PY - 2006
Y1 - 2006
N2 - Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform
AB - Analysing minute amounts of DNA is a routine challenge in forensics in part due to the poor sensitivity of an instrument and its inability to detect results from forensic samples. In this study, the sensitivity of the Pyrosequencing method is investigated using varying concentrations of DNA and five autosomal single nucleotide polymorphisms in singleplex on both available instrument models; the PSQ(TM) 96MA and PSQ(TM) HS 96A. A detailed comparison of the two models was completed while establishing a lower limit of detection on both instruments to give results supporting the use of Pyrosequencing as a valuable forensic SNP typing platform
U2 - doi:10.1016/j.ics.2005.10.021
DO - doi:10.1016/j.ics.2005.10.021
M3 - Article in proceedings
VL - 1288
T3 - ICS - International Congress Series
SP - 52
EP - 54
BT - Progress in Forensic Genetics 11
PB - Elsevier
T2 - 21st International ISFG Congress
Y2 - 13 September 2005 through 16 September 2005
ER -