A "new" primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen

N Morling; B K Jakobsen; P Platz; L P Ryder; A Svejgaard; M Thomsen

A "new" primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen

N Morling, B K Jakobsen, P Platz, L P Ryder, A Svejgaard, M Thomsen

5 Citations (Scopus)

Abstract

We have recently described a "new" private HLA-DR antigen, DR"LTM", which has a frequency of approximately 0.6% in Danes. Primed Lymphocyte Typing (PLT) cells directed towards DR"LTM"-associated determinants were generated in vitro by haplotype primings in two unrelated families with DR"LTM" positive individuals. Both PLT-cells reacted in parallel and gave positive reactions with (i) two unrelated and (ii) eight related individuals, all of whom were DR"LTM"-positive. Both PLT-cells gave negative reactions with (i) 38 unrelated and (ii) 15 related DR"LTM"-negative individuals. Thus, there was total agreement between the results obtained by HLA-DR typing with the antiserum "LTM" and by PLT-typing with these two haplotype primed PLT-cells. None of the DP"LTM"-positive individuals carried more than one of the antigens HLA-Dw/-DRw/DP1-8 and the local specificity D/DP"H". Accordingly, this "new" PLT-defined antigen, DP"LTM", most probably belongs to the series of HLA-D/DR-associated DP-antigens previously described.

Original language	English
Journal	HLA
Volume	16
Issue number	1
Pages (from-to)	95-104
Number of pages	9
ISSN	2059-2302
Publication status	Published - 1980

Cite this

@article{28c30f70b1c511df825b000ea68e967b,

title = "A {"}new{"} primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen",

abstract = "We have recently described a {"}new{"} private HLA-DR antigen, DR{"}LTM{"}, which has a frequency of approximately 0.6% in Danes. Primed Lymphocyte Typing (PLT) cells directed towards DR{"}LTM{"}-associated determinants were generated in vitro by haplotype primings in two unrelated families with DR{"}LTM{"} positive individuals. Both PLT-cells reacted in parallel and gave positive reactions with (i) two unrelated and (ii) eight related individuals, all of whom were DR{"}LTM{"}-positive. Both PLT-cells gave negative reactions with (i) 38 unrelated and (ii) 15 related DR{"}LTM{"}-negative individuals. Thus, there was total agreement between the results obtained by HLA-DR typing with the antiserum {"}LTM{"} and by PLT-typing with these two haplotype primed PLT-cells. None of the DP{"}LTM{"}-positive individuals carried more than one of the antigens HLA-Dw/-DRw/DP1-8 and the local specificity D/DP{"}H{"}. Accordingly, this {"}new{"} PLT-defined antigen, DP{"}LTM{"}, most probably belongs to the series of HLA-D/DR-associated DP-antigens previously described.",

author = "N Morling and Jakobsen, {B K} and P Platz and Ryder, {L P} and A Svejgaard and M Thomsen",

note = "Keywords: Arthritis, Juvenile Rheumatoid; B-Lymphocytes; Diabetes Mellitus; Epitopes; Female; Haploidy; Histocompatibility Antigens Class II; Histocompatibility Testing; Humans; Male; Multiple Sclerosis; Protein Biosynthesis",

year = "1980",

language = "English",

volume = "16",

pages = "95--104",

journal = "HLA",

issn = "2059-2302",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - A "new" primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen

AU - Morling, N

AU - Jakobsen, B K

AU - Platz, P

AU - Ryder, L P

AU - Svejgaard, A

AU - Thomsen, M

N1 - Keywords: Arthritis, Juvenile Rheumatoid; B-Lymphocytes; Diabetes Mellitus; Epitopes; Female; Haploidy; Histocompatibility Antigens Class II; Histocompatibility Testing; Humans; Male; Multiple Sclerosis; Protein Biosynthesis

PY - 1980

Y1 - 1980

N2 - We have recently described a "new" private HLA-DR antigen, DR"LTM", which has a frequency of approximately 0.6% in Danes. Primed Lymphocyte Typing (PLT) cells directed towards DR"LTM"-associated determinants were generated in vitro by haplotype primings in two unrelated families with DR"LTM" positive individuals. Both PLT-cells reacted in parallel and gave positive reactions with (i) two unrelated and (ii) eight related individuals, all of whom were DR"LTM"-positive. Both PLT-cells gave negative reactions with (i) 38 unrelated and (ii) 15 related DR"LTM"-negative individuals. Thus, there was total agreement between the results obtained by HLA-DR typing with the antiserum "LTM" and by PLT-typing with these two haplotype primed PLT-cells. None of the DP"LTM"-positive individuals carried more than one of the antigens HLA-Dw/-DRw/DP1-8 and the local specificity D/DP"H". Accordingly, this "new" PLT-defined antigen, DP"LTM", most probably belongs to the series of HLA-D/DR-associated DP-antigens previously described.

AB - We have recently described a "new" private HLA-DR antigen, DR"LTM", which has a frequency of approximately 0.6% in Danes. Primed Lymphocyte Typing (PLT) cells directed towards DR"LTM"-associated determinants were generated in vitro by haplotype primings in two unrelated families with DR"LTM" positive individuals. Both PLT-cells reacted in parallel and gave positive reactions with (i) two unrelated and (ii) eight related individuals, all of whom were DR"LTM"-positive. Both PLT-cells gave negative reactions with (i) 38 unrelated and (ii) 15 related DR"LTM"-negative individuals. Thus, there was total agreement between the results obtained by HLA-DR typing with the antiserum "LTM" and by PLT-typing with these two haplotype primed PLT-cells. None of the DP"LTM"-positive individuals carried more than one of the antigens HLA-Dw/-DRw/DP1-8 and the local specificity D/DP"H". Accordingly, this "new" PLT-defined antigen, DP"LTM", most probably belongs to the series of HLA-D/DR-associated DP-antigens previously described.

M3 - Journal article

C2 - 6162231

SN - 2059-2302

VL - 16

SP - 95

EP - 104

JO - HLA

JF - HLA

IS - 1

ER -

A "new" primed lymphocyte typing (PLT) defined DP-antigen associated with a private HLA--DR antigen

Abstract

Fingerprint

Cite this